Diabetes decreases mRNA levels of calcium-release channels in human atrial appendage

Sahika Guner, Ebru Arioglu, Aydin Tay, Atalay Tasdelen, Sait Aslamaci, Keshore R. Bidasee, Umit Deniz Dincer

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Patients with chronic diabetes mellitus usually develop reductions in rate and force of cardiac contractions. Since calcium-release channels (ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs)) play integral roles in effecting these processes, we rationalize that alterations in their expression may underlie these defects. To test this hypothesis, right atrial appendages were obtained from diabetic (65.0 ± 4.5 years) and nondiabetic (56.2 ± 2.6 years) patients undergoing coronary arterial by-pass grafting and reverse transcription-polymerase chain reactions were used to compare steady state levels of mRNA encoding the three major isoforms of RyRs and IP3Rs. In this study we did not detect either RyR1 or RyR3 in human atrial appendage. When compared with nondiabetic patients, mRNA encoding RyR2 from diabetic patients decreased by 74.2 ± 6.2% (p < 0.01). Diabetes also significantly decreased steady-state levels of mRNA encoding the IP3Rs in human atrial appendage. IP3R1 decreased by 24.2 ± 4.6%, IP3R2 decreased by 63.0 ± 4.6% and IP3R3 decreased by 55.5 ± 6.5%. Since a reduction in steady-state mRNA is usually indicative of a decrease in protein levels, these data suggest that the decrease in chronotropy and inotropy seen in chronic diabetic patients may be due in part to a decrease in expression of calcium-release channels.

Original languageEnglish (US)
Pages (from-to)143-150
Number of pages8
JournalMolecular and cellular biochemistry
Volume263
Issue number1
DOIs
StatePublished - Aug 2004

Keywords

  • 1,4,5-trisphosphate receptors
  • Calcium-release channels
  • Diabetes
  • Expression
  • Human atrial appendage
  • Inositol
  • Reverse transcription-polymerase chain reaction
  • Ryanodine receptors

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Diabetes decreases mRNA levels of calcium-release channels in human atrial appendage'. Together they form a unique fingerprint.

  • Cite this