Differences in molecular biological, biological and growth characteristics between the immortal and malignant hamster pancreatic cells

Toshiyuki Takahashi, Mary P. Moyer, Martin Cano, Qiming J. Wang, Charles P. Mountjoy, Warren Sanger, Thomas E. Adrian, Hiroshi Sugiura, Hiroyuki Katoh, Parviz M. Pour

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We compared morphological, biological and molecular biological patterns of a newly established, spontaneously immortalized pancreatic ductal cell line, TAKA-1, with a hamster pancreatic ductal adenocarcinoma cell line, PC-1. PC-1 cells grew in a monolayer on plastic tissue culture flasks, whereas TAKA-1 cells required type I collagen gel matrix to propagate. The growth rate and argyrophilic nuclear organizer region (Ag-NOR) counts were greater in PC-1 cells than in TAKA-1 cells. More TAKA-1 cells were in G0/G1 and less were in the S cell cycle phase than PC-1 cells. Karyotypically, the consistent change in TAKA-1 cells was an abnormal no. 3 chromosome, whereas additional chromosomal abnormalities were found in PC-1 cells. Ultrastructurally, TAKA-1 cells formed ductal structures and were composed of two types of cells, as in the normal hamster pancreatic ducts, whereas PC-1 cells were pleomorphic, showed evidence for loss of differentiation and contained intracytoplasmic lumens. Unlike the PC-1, TAKA-1 cells did not show a point mutation at codon 12 in the c-Ki-ras oncogene and did not grow in soft agar. Receptor binding assay showed specific epidermal growth factor binding to both cell lines, but secretin binding only to TAKA-1 cells. Both cells produced and released transforming growth factor-α in serum-free medium. Both cell lines expressed blood group A antigen, carbonic anhydrase, coexpressed cytokeratin and vimentin, and reacted with tomato and Phaseolus vulgaris leucoagglutinin (L-PHA) lectins. The results demonstrate that chromosomal abnormalities, cell cycle patterns, expression of cytokeratin 18, lectin bindings and the c-Ki-ras mutation are the features that distinguish the benign from the malignant pancreatic ductal cells in Syrian hamster.

Original languageEnglish (US)
Pages (from-to)931-939
Number of pages9
JournalCarcinogenesis
Volume16
Issue number4
DOIs
StatePublished - Apr 1995

ASJC Scopus subject areas

  • Cancer Research

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    Takahashi, T., Moyer, M. P., Cano, M., Wang, Q. J., Mountjoy, C. P., Sanger, W., Adrian, T. E., Sugiura, H., Katoh, H., & Pour, P. M. (1995). Differences in molecular biological, biological and growth characteristics between the immortal and malignant hamster pancreatic cells. Carcinogenesis, 16(4), 931-939. https://doi.org/10.1093/carcin/16.4.931