Long-chain bases, found in the oral cavity, have potent antimicrobial activity against oral pathogens. In an article associated with this dataset, Poulson and colleagues determined the cytotoxicities of long-chain bases (sphingosine, dihydrosphingosine, and phytosphingosine) for human oral gingival epithelial (GE) keratinocytes, oral gingival fibroblasts (GF), dendritic cells (DC), and squamous cell carcinoma (SCC) cell lines . Poulson and colleagues found that GE keratinocytes were more resistant to long-chain bases as compared to GF, DC, and SCC cell lines . In this study, we assess the susceptibility of DC to lower concentrations of long chain bases. 0.2-10.0μM long-chain bases and GML were not cytotoxic to DC; 40.0-80.0μM long-chain bases, but not GML, were cytotoxic for DC; and 80.0μM long-chain bases were cytotoxic to DC and induced cellular damage and death in less than 20mins. Overall, the LD50 of long-chain bases for GE keratinocytes, GF, and DC were considerably higher than their minimal inhibitory concentrations for oral pathogens, a finding important to pursuing their future potential in treating periodontal and oral infections.
- Dendritic cells
- Glycerol monolaurate
- Human oral gingival epithelial keratinocytes
- Oral gingival fibroblasts
- Oral squamous cell carcinoma cells
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