Differential effects of eicosapentaenoic acid and docosahexaenoic acid in promoting the differentiation of 3T3-L1 preadipocytes

Ganesan Murali, Cyrus V. Desouza, Michelle E. Clevenger, Ramesh Ramalingam, Viswanathan Saraswathi

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The objective of this study was to determine the effects of enrichment with n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the differentiation of 3T3-L1 preadipocytes. Enrichment with DHA but not EPA significantly increased the differentiation markers compared to control differentiated cells. DHA compared to EPA treatment led to a greater increase in adiponectin secretion and, conditioned media collected from DHA treated cells inhibited monocyte migration. Moreover, DHA treatment resulted in inhibition of pro-inflammatory signaling pathways. DHA treated cells predominantly accumulated DHA in phospholipids whereas EPA treatment led to accumulation of both EPA and its elongation product docosapentaenoic acid (DPA), an n-3 fatty acid. Of note, adding DPA to DHA inhibited DHA-induced differentiation. The differential effects of EPA and DHA on preadipocyte differentiation may be due, in part, to differences in their intracellular modification which could impact the type of n-3 fatty acids incorporated into the cells.

Original languageEnglish (US)
Pages (from-to)13-21
Number of pages9
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume90
Issue number1
DOIs
StatePublished - Jan 2014

Keywords

  • Adipogenesis
  • DHA
  • Docosapentaenoic acid
  • EPA
  • N-3 fatty acids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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