TY - JOUR
T1 - Differential effects of eicosapentaenoic acid and docosahexaenoic acid in promoting the differentiation of 3T3-L1 preadipocytes
AU - Murali, Ganesan
AU - Desouza, Cyrus V.
AU - Clevenger, Michelle E.
AU - Ramalingam, Ramesh
AU - Saraswathi, Viswanathan
N1 - Funding Information:
This project was supported in part, by the American Heart Association Scientist Development Grant ( 0930335N ) to V. Saraswathi. The work was conducted using the facilities and resources at the VA Nebraska Western Iowa Health Care System, Omaha. Fatty acid analysis was performed at the Lipid Core Laboratory of the Mouse Metabolic Phenotyping Center at Vanderbilt University ( DK59637 ).
PY - 2014/1
Y1 - 2014/1
N2 - The objective of this study was to determine the effects of enrichment with n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the differentiation of 3T3-L1 preadipocytes. Enrichment with DHA but not EPA significantly increased the differentiation markers compared to control differentiated cells. DHA compared to EPA treatment led to a greater increase in adiponectin secretion and, conditioned media collected from DHA treated cells inhibited monocyte migration. Moreover, DHA treatment resulted in inhibition of pro-inflammatory signaling pathways. DHA treated cells predominantly accumulated DHA in phospholipids whereas EPA treatment led to accumulation of both EPA and its elongation product docosapentaenoic acid (DPA), an n-3 fatty acid. Of note, adding DPA to DHA inhibited DHA-induced differentiation. The differential effects of EPA and DHA on preadipocyte differentiation may be due, in part, to differences in their intracellular modification which could impact the type of n-3 fatty acids incorporated into the cells.
AB - The objective of this study was to determine the effects of enrichment with n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the differentiation of 3T3-L1 preadipocytes. Enrichment with DHA but not EPA significantly increased the differentiation markers compared to control differentiated cells. DHA compared to EPA treatment led to a greater increase in adiponectin secretion and, conditioned media collected from DHA treated cells inhibited monocyte migration. Moreover, DHA treatment resulted in inhibition of pro-inflammatory signaling pathways. DHA treated cells predominantly accumulated DHA in phospholipids whereas EPA treatment led to accumulation of both EPA and its elongation product docosapentaenoic acid (DPA), an n-3 fatty acid. Of note, adding DPA to DHA inhibited DHA-induced differentiation. The differential effects of EPA and DHA on preadipocyte differentiation may be due, in part, to differences in their intracellular modification which could impact the type of n-3 fatty acids incorporated into the cells.
KW - Adipogenesis
KW - DHA
KW - Docosapentaenoic acid
KW - EPA
KW - N-3 fatty acids
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U2 - 10.1016/j.plefa.2013.10.002
DO - 10.1016/j.plefa.2013.10.002
M3 - Article
C2 - 24332315
AN - SCOPUS:84891153713
VL - 90
SP - 13
EP - 21
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
SN - 0952-3278
IS - 1
ER -