Differential expression of mads-box genes in the morphogenesis of calli in rice

Guang Qing Wang, Jian Guang Hu, Xiang Shan Zhao, Ning Hui Cheng, Xiao Yin Qian, Jin Shui Yang

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Total RNAs from the suspension cell line, callus, redifferentiatied callus and regenerat-ed plantlets of rice ( Oryza sativa L. ssp. indica ) "Zhenshan 97B" were reverse-transcribed with poly (T) primers. The cDNAs were amplified by a pair of poly (T) primers and MADS (MCM1-Agamous-Deficiens-SRF) domain-specific primers and then separated on a sequencing gel. Several differentially expressed cDNA fragments were isolated. Sequencing of RM1, one of the differentially expressed gene cDNA fragments, showed that it contained a MADS domain, the primary structure of which shared a 63% homology with the typical MADS-box agamous protein of Arabidopsis thaliana. Computer analysis also indicated that the secondary structure of RM1 peptide was significantly similar to that of agamous protein. It was shown that RM1 belonged to the MADS-box gene family. Molecular hybridization showed that RM1 was silent in the suspension cells, while active in the callus, redifferentiated callus and regenerated plantlets.

Original languageEnglish (US)
Pages (from-to)1035-1041
Number of pages7
JournalActa Botanica Sinica
Volume39
Issue number11
StatePublished - Dec 1 1997

Keywords

  • Cell differentiation
  • MADS domain
  • Rice
  • mRNA differential display

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

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    Wang, G. Q., Hu, J. G., Zhao, X. S., Cheng, N. H., Qian, X. Y., & Yang, J. S. (1997). Differential expression of mads-box genes in the morphogenesis of calli in rice. Acta Botanica Sinica, 39(11), 1035-1041.