TY - JOUR
T1 - Differential gene expression related to Nora virus infection of Drosophila melanogaster
AU - Cordes, Ethan J.
AU - Licking-Murray, Kellie D.
AU - Carlson, Kimberly A.
N1 - Funding Information:
We thank Dr. Jim Eudy for cDNA microarray hybridizations and Dr. Lynette Smith for the statistical analysis. Drs. Hultmark and Ekström for their gift of the original Nora virus stocks and collaboration throughout the project. Also, Dr. Brad Ericson for critical review and editing of the manuscript. The project described was supported by grants from the National Center for Research Resources (NCRR; 5P20RR016469 ) and the National Institute for General Medical Science (NIGMS; 8P20GM103427 ), a component of the National Institutes of Health (NIH) and RSC grant from the University of Nebraska at Kearney . The UNMC Microarray Core receives partial support from the NCRR (5P20RR016469, RR018788-08 ) and NIGMS (8P20GM103427, GM103471-09 ). This publication's contents are the sole responsibility of the authors and do not necessarily represent the official views of the NIH or NIGMS.
PY - 2013/8
Y1 - 2013/8
N2 - Nora virus is a recently discovered RNA picorna-like virus that produces a persistent infection in Drosophila melanogaster, but the antiviral pathway or change in gene expression is unknown. We performed cDNA microarray analysis comparing the gene expression profiles of Nora virus infected and uninfected wild-type D. melanogaster. This analysis yielded 58 genes exhibiting a 1.5-fold change or greater and p-value less than 0.01. Of these genes, 46 were up-regulated and 12 down-regulated in response to infection. To validate the microarray results, qRT-PCR was performed with probes for Chorion protein 16 and Troponin C isoform 4, which show good correspondence with cDNA microarray results. Differential regulation of genes associated with Toll and immune-deficient pathways, cytoskeletal development, Janus Kinase-Signal Transducer and Activator of Transcription interactions, and a potential gut-specific innate immune response were found. This genome-wide expression profile of Nora virus infection of D. melanogaster can pinpoint genes of interest for further investigation of antiviral pathways employed, genetic mechanisms, sites of replication, viral persistence, and developmental effects.
AB - Nora virus is a recently discovered RNA picorna-like virus that produces a persistent infection in Drosophila melanogaster, but the antiviral pathway or change in gene expression is unknown. We performed cDNA microarray analysis comparing the gene expression profiles of Nora virus infected and uninfected wild-type D. melanogaster. This analysis yielded 58 genes exhibiting a 1.5-fold change or greater and p-value less than 0.01. Of these genes, 46 were up-regulated and 12 down-regulated in response to infection. To validate the microarray results, qRT-PCR was performed with probes for Chorion protein 16 and Troponin C isoform 4, which show good correspondence with cDNA microarray results. Differential regulation of genes associated with Toll and immune-deficient pathways, cytoskeletal development, Janus Kinase-Signal Transducer and Activator of Transcription interactions, and a potential gut-specific innate immune response were found. This genome-wide expression profile of Nora virus infection of D. melanogaster can pinpoint genes of interest for further investigation of antiviral pathways employed, genetic mechanisms, sites of replication, viral persistence, and developmental effects.
KW - CDNA microarray
KW - Gut immunity
KW - Nora virus
KW - RNA viruses
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U2 - 10.1016/j.virusres.2013.03.021
DO - 10.1016/j.virusres.2013.03.021
M3 - Article
C2 - 23603562
AN - SCOPUS:84878882095
SN - 0168-1702
VL - 175
SP - 95
EP - 100
JO - Virus Research
JF - Virus Research
IS - 2
ER -