Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epithelium

Margaret Elliott; Joseph Sisson; William West; Todd Wyatt

doi:10.1080/01902140600710546

Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epithelium

Margaret Elliott, Joseph Sisson, William West, Todd Wyatt

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

In this study the authors compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. The authors found that CSE treatment significantly increased neutrophil influx (P < .001), baseline ciliary beat frequency (CBF) (P < .05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE (P < .01). Isoproterenol increased protein kinase A (PKA) activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.

Original language	English (US)
Pages (from-to)	99-118
Number of pages	20
Journal	Experimental Lung Research
Volume	32
Issue number	3-4
DOIs	https://doi.org/10.1080/01902140600710546
State	Published - Jul 2006

Keywords

Cigarette smoke
Cigarette smoke extract
Cilia beat frequency
Mouse model
SAVA
Tracheal epithelium

ASJC Scopus subject areas

Molecular Biology
Pulmonary and Respiratory Medicine
Clinical Biochemistry

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10.1080/01902140600710546

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title = "Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epithelium",

abstract = "In this study the authors compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. The authors found that CSE treatment significantly increased neutrophil influx (P < .001), baseline ciliary beat frequency (CBF) (P < .05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE (P < .01). Isoproterenol increased protein kinase A (PKA) activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.",

keywords = "Cigarette smoke, Cigarette smoke extract, Cilia beat frequency, Mouse model, SAVA, Tracheal epithelium",

author = "Margaret Elliott and Joseph Sisson and William West and Todd Wyatt",

note = "Funding Information: Received 17 October 2005; accepted 27 February 2006. This work was supported by 5T32AA007582-05 (MKE), 5R01AA008769-15 (JHS), and Veterans Administration Merit Review Award (TAW). Dr. Wyatt is an American Lung Association Career Investigator. Address correspondence to Todd A. Wyatt, PhD, University of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198-5300, USA. E-mail: twyatt@unmc.edu",

year = "2006",

month = jul,

doi = "10.1080/01902140600710546",

language = "English (US)",

volume = "32",

pages = "99--118",

journal = "Experimental Lung Research",

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AU - Elliott, Margaret

AU - Sisson, Joseph

AU - West, William

AU - Wyatt, Todd

N1 - Funding Information: Received 17 October 2005; accepted 27 February 2006. This work was supported by 5T32AA007582-05 (MKE), 5R01AA008769-15 (JHS), and Veterans Administration Merit Review Award (TAW). Dr. Wyatt is an American Lung Association Career Investigator. Address correspondence to Todd A. Wyatt, PhD, University of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198-5300, USA. E-mail: twyatt@unmc.edu

PY - 2006/7

Y1 - 2006/7

N2 - In this study the authors compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. The authors found that CSE treatment significantly increased neutrophil influx (P < .001), baseline ciliary beat frequency (CBF) (P < .05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE (P < .01). Isoproterenol increased protein kinase A (PKA) activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.

AB - In this study the authors compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. The authors found that CSE treatment significantly increased neutrophil influx (P < .001), baseline ciliary beat frequency (CBF) (P < .05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE (P < .01). Isoproterenol increased protein kinase A (PKA) activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.

KW - Cigarette smoke

KW - Cigarette smoke extract

KW - Cilia beat frequency

KW - Mouse model

KW - SAVA

KW - Tracheal epithelium

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SN - 0190-2148

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JO - Experimental Lung Research

JF - Experimental Lung Research

IS - 3-4

ER -

Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epithelium

Abstract

Keywords

ASJC Scopus subject areas

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