TY - JOUR
T1 - Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epithelium
AU - Elliott, Margaret
AU - Sisson, Joseph
AU - West, William
AU - Wyatt, Todd
N1 - Funding Information:
Received 17 October 2005; accepted 27 February 2006. This work was supported by 5T32AA007582-05 (MKE), 5R01AA008769-15 (JHS), and Veterans Administration Merit Review Award (TAW). Dr. Wyatt is an American Lung Association Career Investigator. Address correspondence to Todd A. Wyatt, PhD, University of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198-5300, USA. E-mail: [email protected]
PY - 2006/7
Y1 - 2006/7
N2 - In this study the authors compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. The authors found that CSE treatment significantly increased neutrophil influx (P < .001), baseline ciliary beat frequency (CBF) (P < .05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE (P < .01). Isoproterenol increased protein kinase A (PKA) activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.
AB - In this study the authors compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. The authors found that CSE treatment significantly increased neutrophil influx (P < .001), baseline ciliary beat frequency (CBF) (P < .05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE (P < .01). Isoproterenol increased protein kinase A (PKA) activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.
KW - Cigarette smoke
KW - Cigarette smoke extract
KW - Cilia beat frequency
KW - Mouse model
KW - SAVA
KW - Tracheal epithelium
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U2 - 10.1080/01902140600710546
DO - 10.1080/01902140600710546
M3 - Article
C2 - 16754475
AN - SCOPUS:33745008642
SN - 0190-2148
VL - 32
SP - 99
EP - 118
JO - Experimental Lung Research
JF - Experimental Lung Research
IS - 3-4
ER -