TY - JOUR
T1 - Differential regulation of the transforming growth factor type-β2 gene promoter in embryonal carcinoma cells and their differentiated cells
AU - Kelly, David
AU - O'Reilly, Michael A.
AU - Rizzino, Angie
N1 - Funding Information:
Solon Rhode is thanked for his advice during the early stages of this work. Keith Miller and Phillip Wilder are thanked for their comments on this manuscript. This work was supported bg a grant from the Council of Tobacco Research (2.520)a nd by core grants from the National Cancer Institute (Laboratory Cancer Research Center Support Grant, CA 36727) and the American Cancer Society (ACS SIG-16). David Kelly was supported by a graduate fellowship provided by the Nebraska Research Initiative in Biotechnology.
PY - 1992/9
Y1 - 1992/9
N2 - Previous studies have shown that EC cells do not express detectable levels of TGF-β2 or its mRNA until they differentiate. This suggested that differentiation influences the transcription of the TGF-β2 gene in this model system. To address this possibility, we have examined the activity of the TGF-β2 promoter in EC cells and their differentiated cells using gene constructs containing various portions of the TGF-β2 promoter inserted upstream of the reporter gene, chloramphenicol acetyltransferase (CAT). We determined that the level of CAT increases approximately ninefold when EC cells were induced to differentiate. Our studies also indicate that the TGF-β2 promoter contains at least two positive regulatory elements that are separated by a negative regulatory element. Finally, we have identified a CRE ATF-like site that appears to be responsible for a positive regulatory element located between -77 and -40.
AB - Previous studies have shown that EC cells do not express detectable levels of TGF-β2 or its mRNA until they differentiate. This suggested that differentiation influences the transcription of the TGF-β2 gene in this model system. To address this possibility, we have examined the activity of the TGF-β2 promoter in EC cells and their differentiated cells using gene constructs containing various portions of the TGF-β2 promoter inserted upstream of the reporter gene, chloramphenicol acetyltransferase (CAT). We determined that the level of CAT increases approximately ninefold when EC cells were induced to differentiate. Our studies also indicate that the TGF-β2 promoter contains at least two positive regulatory elements that are separated by a negative regulatory element. Finally, we have identified a CRE ATF-like site that appears to be responsible for a positive regulatory element located between -77 and -40.
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U2 - 10.1016/0012-1606(92)90102-M
DO - 10.1016/0012-1606(92)90102-M
M3 - Article
C2 - 1516748
AN - SCOPUS:0026661231
SN - 0012-1606
VL - 153
SP - 172
EP - 175
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -