Abstract
The susceptibility of metastatic variant lymphoma cells to natural immunity was studied using a low malignant/metastatic parental RAW117-P cell line and its liver colonizing highly malignant/metastatic RAW117-H10 cell line. The metastatic variant RAW117-H10 cells express a significantly lower amount of laminin-like and fibronectin-like molecules as determined by flow cytometry using monospecific polyclonal antibodies to laminin and fibronectin. Our studies indicated that the RAW117-H10 cells are resistant to natural killer (NK) cell-mediated cytotoxicity. In vitro activation of the effector cells with interferon-gamma increased the susceptibility of these cells to NK-mediated cytotoxicity while maintaining the difference between the two cell lines. However, when recombinant interleukin-2 was used to activate the effector cells, the cytotoxicity of the lymphokine-activated effector cells to both parental low metastatic RAW117-P cells and highly metastatic RAW117-H10 cells was similar.
Original language | English (US) |
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Pages (from-to) | 483-487 |
Number of pages | 5 |
Journal | ONCOLOGY |
Volume | 47 |
Issue number | 6 |
DOIs | |
State | Published - 1990 |
Keywords
- cell surface glycoconjugates
- lymphokine-activated killer cells
- metastasis
- murine lymphoma
- natural immunity
- natural killer cells
ASJC Scopus subject areas
- Oncology
- Cancer Research