Differentiation of embryonic stem cells into retinal neurons

Xing Zhao, Jianuo Liu, Iqbal Ahmad

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

Mouse embryonic stem (ES) cells are continuous cell lines derived from the inner mass of blastocysts. Neural progenitors derived from these cells serve as an excellent model for controlled neural differentiation and as such have tremendous potential to understand and treat neurodegenerative diseases. Here, we demonstrate that ES cell-derived neural progenitors express regulatory factors needed for retinal differentiation and that in response to epigenetic cues a subset of them differentiate along photoreceptor lineage. During the differentiation, they activate photoreceptor regulatory genes, suggesting that ES cell-derived neural progenitors recruit mechanisms normally used for photoreceptor differentiation in vivo. These observations suggest that ES cells can serve as an excellent model for understanding mechanisms that regulate specification of retinal neurons and as an unlimited source of neural progenitors for treating degenerative diseases of the retina by cell replacement.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume297
Issue number2
DOIs
StatePublished - Nov 6 2002

Keywords

  • Bipolar cells
  • Differentiation
  • ES cells
  • Eye
  • Photoreceptors
  • Retina
  • Stem cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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