TY - JOUR
T1 - Diminished matrix metalloproteinase 9 secretion in human immunodeficiency virus-infected mononuclear phagocytes
T2 - Modulation of innate immunity and implications for neurological disease
AU - Ciborowski, Pawel
AU - Enose, Yoshimi
AU - MacK, Andrea
AU - Fladseth, Melissa
AU - Gendelman, Howard E.
N1 - Funding Information:
The work was supported by NIH Grants 5 R37 NS36126-07, 5 R01 NS034239-10, 1 P01 NS43985-01A1, 5 P01NS31492-11, 5 R01 MH64570-03, P01 NS11766-28, 20 RR15635 from the COBRE Program of the National Center for Research Resources. The authors extend a special thanks to Ms. Robin Taylor for outstanding administrative and computer support and Ms. Lisa Walters for technical help with RT assay.
PY - 2004/12
Y1 - 2004/12
N2 - Neurotoxic secretory products from virus-infected mononuclear phagocytes (MP; perivascular macrophages and microglia) orchestrate the neuropathogenesis of human immunodeficiency virus type one (HIV-1) infection. To uncover such MP products and their relationship to disease, we used a proteomics platform consisting of one dimensional polyacrylamide gel electrophoresis (1-DE), mass spectrometry peptide sequencing, and bioinformatics in order to identify from HIV-1-infected monocyte-derived macrophages (MDM) secretions. Matrix metalloproteinase 9 (MMP 9) secreted in abundance in MDM was markedly down-regulated following viral infection. A negative correlation between MMP 9 and HIV-1 reverse transcriptase activity was shown by quantitative Western blot assays. These data further demonstrate immunoregulatory activities of HIV-1-infected MDM providing unique insights into cellular function in disease.
AB - Neurotoxic secretory products from virus-infected mononuclear phagocytes (MP; perivascular macrophages and microglia) orchestrate the neuropathogenesis of human immunodeficiency virus type one (HIV-1) infection. To uncover such MP products and their relationship to disease, we used a proteomics platform consisting of one dimensional polyacrylamide gel electrophoresis (1-DE), mass spectrometry peptide sequencing, and bioinformatics in order to identify from HIV-1-infected monocyte-derived macrophages (MDM) secretions. Matrix metalloproteinase 9 (MMP 9) secreted in abundance in MDM was markedly down-regulated following viral infection. A negative correlation between MMP 9 and HIV-1 reverse transcriptase activity was shown by quantitative Western blot assays. These data further demonstrate immunoregulatory activities of HIV-1-infected MDM providing unique insights into cellular function in disease.
KW - HIV-1-associated dementia
KW - Human immunodeficiency virus
KW - Matrix metalloproteinases
KW - Monocyte-derived macrophages
KW - Proteomics
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U2 - 10.1016/j.jneuroim.2004.08.033
DO - 10.1016/j.jneuroim.2004.08.033
M3 - Article
C2 - 15579275
AN - SCOPUS:9644289505
VL - 157
SP - 11
EP - 16
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1-2 SPEC. ISS.
ER -