Original language | English (US) |
---|---|
Pages (from-to) | 822 |
Number of pages | 1 |
Journal | Human Molecular Genetics |
Volume | 2 |
Issue number | 6 |
DOIs |
|
State | Published - Jun 1993 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Genetics(clinical)
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In: Human Molecular Genetics, Vol. 2, No. 6, 06.1993, p. 822.
Research output: Contribution to journal › Comment/debate › peer-review
}
TY - JOUR
T1 - Dinucleotide repeat polymorphism at the human olfactory marker protein (OMP) locus on chromosome 11q13.5 near tyrosinase (TYR)
AU - Dahl, S. Pieke
AU - Weston, M. D.
AU - Overbeck, L. D.
AU - Evans, K. L.
AU - Porteous, D. J.
AU - Kimberling, W. J.
N1 - Funding Information: Acknowledgement: The support of a Telethon Italy grant to M.P. is gratefully acknowledged. References: 1) Chamberlain,S. et al. (1993) Amer. J. Hum. Genet., 52, 99-109. 2) Sirugo.G. et al. (1993) Europ. J. Hum. Genet., 1, 133-143. Funding Information: PCR Conditions: PCRs were done in 25 /tl with 20 ng genomic DNA, 1.5 mM MgCl2, 10 mM Tris-Cl pH 8.3, 50 mM KC1, 0.01 % gelatin (Promega lOx Taq polymerase buffer), 200 /iM each of dCTP.dGTP, dTTP, and 2.5 /tM dATP, 1 /tCi a P32 dATP (NEN Du Pont, 800 Ci/mmol, 10 /*Ci//*l), 10 ng each oligonucleotide primer, 1 unit of Taq DNA polymerase (Promega). PCR conditions were set at denaturation at 94°C for 1 min., annealing at 55°C for 1 min., and extension at 72°C for 2 min. for 29 cycles. A final cycle ended with 10 min. of extension at 72°C. Fragments were separated on 6% polyacrylamide denaturing gels. Dideoxy sequencing ladders of M13mpl8 DNA were used as fragment size standards. Other Comments: The human OMP sequence has been assigned EMBL accession number Z18943. Acknowledgements: Supported by NTH grants RO1DC00677, grant 89-198 from the Retinitis Pigmentosa Foundation, and the UK Human Genome Mapping Programme. References: 1) Brown.K.A. etal. (1992) Genomics 13, 189-193. 2) Evans.K.L. et al. (1993) Hum. Mol. Genet, in press.
PY - 1993/6
Y1 - 1993/6
UR - http://www.scopus.com/inward/record.url?scp=0027175505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027175505&partnerID=8YFLogxK
U2 - 10.1093/hmg/2.6.822-a
DO - 10.1093/hmg/2.6.822-a
M3 - Comment/debate
C2 - 8353507
AN - SCOPUS:0027175505
SN - 0964-6906
VL - 2
SP - 822
JO - Human Molecular Genetics
JF - Human Molecular Genetics
IS - 6
ER -