@article{50b7b0e084c94e6da1f04583cb9d1dfb,
title = "Dinucleotide repeat polymorphism at the human olfactory marker protein (OMP) locus on chromosome 11q13.5 near tyrosinase (TYR)",
author = "Dahl, {S. Pieke} and Weston, {M. D.} and Overbeck, {L. D.} and Evans, {K. L.} and Porteous, {D. J.} and Kimberling, {W. J.}",
note = "Funding Information: Acknowledgement: The support of a Telethon Italy grant to M.P. is gratefully acknowledged. References: 1) Chamberlain,S. et al. (1993) Amer. J. Hum. Genet., 52, 99-109. 2) Sirugo.G. et al. (1993) Europ. J. Hum. Genet., 1, 133-143. Funding Information: PCR Conditions: PCRs were done in 25 /tl with 20 ng genomic DNA, 1.5 mM MgCl2, 10 mM Tris-Cl pH 8.3, 50 mM KC1, 0.01 % gelatin (Promega lOx Taq polymerase buffer), 200 /iM each of dCTP.dGTP, dTTP, and 2.5 /tM dATP, 1 /tCi a P32 dATP (NEN Du Pont, 800 Ci/mmol, 10 /*Ci//*l), 10 ng each oligonucleotide primer, 1 unit of Taq DNA polymerase (Promega). PCR conditions were set at denaturation at 94°C for 1 min., annealing at 55°C for 1 min., and extension at 72°C for 2 min. for 29 cycles. A final cycle ended with 10 min. of extension at 72°C. Fragments were separated on 6% polyacrylamide denaturing gels. Dideoxy sequencing ladders of M13mpl8 DNA were used as fragment size standards. Other Comments: The human OMP sequence has been assigned EMBL accession number Z18943. Acknowledgements: Supported by NTH grants RO1DC00677, grant 89-198 from the Retinitis Pigmentosa Foundation, and the UK Human Genome Mapping Programme. References: 1) Brown.K.A. etal. (1992) Genomics 13, 189-193. 2) Evans.K.L. et al. (1993) Hum. Mol. Genet, in press.",
year = "1993",
month = jun,
doi = "10.1093/hmg/2.6.822-a",
language = "English (US)",
volume = "2",
pages = "822",
journal = "Human Molecular Genetics",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "6",
}