Dinucleotide repeat polymorphism at the human olfactory marker protein (OMP) locus on chromosome 11q13.5 near tyrosinase (TYR)

S. Pieke Dahl; M. D. Weston; L. D. Overbeck; K. L. Evans; D. J. Porteous; W. J. Kimberling

doi:10.1093/hmg/2.6.822-a

Dinucleotide repeat polymorphism at the human olfactory marker protein (OMP) locus on chromosome 11q13.5 near tyrosinase (TYR)

S. Pieke Dahl, M. D. Weston, L. D. Overbeck, K. L. Evans, D. J. Porteous, W. J. Kimberling

Research output: Contribution to journal › Comment/debate › peer-review

Original language	English (US)
Pages (from-to)	822
Number of pages	1
Journal	Human Molecular Genetics
Volume	2
Issue number	6
DOIs	https://doi.org/10.1093/hmg/2.6.822-a
State	Published - Jun 1993
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Genetics(clinical)

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10.1093/hmg/2.6.822-a

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@article{50b7b0e084c94e6da1f04583cb9d1dfb,

title = "Dinucleotide repeat polymorphism at the human olfactory marker protein (OMP) locus on chromosome 11q13.5 near tyrosinase (TYR)",

author = "Dahl, {S. Pieke} and Weston, {M. D.} and Overbeck, {L. D.} and Evans, {K. L.} and Porteous, {D. J.} and Kimberling, {W. J.}",

note = "Funding Information: Acknowledgement: The support of a Telethon Italy grant to M.P. is gratefully acknowledged. References: 1) Chamberlain,S. et al. (1993) Amer. J. Hum. Genet., 52, 99-109. 2) Sirugo.G. et al. (1993) Europ. J. Hum. Genet., 1, 133-143. Funding Information: PCR Conditions: PCRs were done in 25 /tl with 20 ng genomic DNA, 1.5 mM MgCl2, 10 mM Tris-Cl pH 8.3, 50 mM KC1, 0.01 % gelatin (Promega lOx Taq polymerase buffer), 200 /iM each of dCTP.dGTP, dTTP, and 2.5 /tM dATP, 1 /tCi a P32 dATP (NEN Du Pont, 800 Ci/mmol, 10 /*Ci//*l), 10 ng each oligonucleotide primer, 1 unit of Taq DNA polymerase (Promega). PCR conditions were set at denaturation at 94°C for 1 min., annealing at 55°C for 1 min., and extension at 72°C for 2 min. for 29 cycles. A final cycle ended with 10 min. of extension at 72°C. Fragments were separated on 6% polyacrylamide denaturing gels. Dideoxy sequencing ladders of M13mpl8 DNA were used as fragment size standards. Other Comments: The human OMP sequence has been assigned EMBL accession number Z18943. Acknowledgements: Supported by NTH grants RO1DC00677, grant 89-198 from the Retinitis Pigmentosa Foundation, and the UK Human Genome Mapping Programme. References: 1) Brown.K.A. etal. (1992) Genomics 13, 189-193. 2) Evans.K.L. et al. (1993) Hum. Mol. Genet, in press.",

year = "1993",

month = jun,

doi = "10.1093/hmg/2.6.822-a",

language = "English (US)",

volume = "2",

pages = "822",

journal = "Human Molecular Genetics",

issn = "0964-6906",

publisher = "Oxford University Press",

number = "6",

}

TY - JOUR

T1 - Dinucleotide repeat polymorphism at the human olfactory marker protein (OMP) locus on chromosome 11q13.5 near tyrosinase (TYR)

AU - Dahl, S. Pieke

AU - Weston, M. D.

AU - Overbeck, L. D.

AU - Evans, K. L.

AU - Porteous, D. J.

AU - Kimberling, W. J.

N1 - Funding Information: Acknowledgement: The support of a Telethon Italy grant to M.P. is gratefully acknowledged. References: 1) Chamberlain,S. et al. (1993) Amer. J. Hum. Genet., 52, 99-109. 2) Sirugo.G. et al. (1993) Europ. J. Hum. Genet., 1, 133-143. Funding Information: PCR Conditions: PCRs were done in 25 /tl with 20 ng genomic DNA, 1.5 mM MgCl2, 10 mM Tris-Cl pH 8.3, 50 mM KC1, 0.01 % gelatin (Promega lOx Taq polymerase buffer), 200 /iM each of dCTP.dGTP, dTTP, and 2.5 /tM dATP, 1 /tCi a P32 dATP (NEN Du Pont, 800 Ci/mmol, 10 /*Ci//*l), 10 ng each oligonucleotide primer, 1 unit of Taq DNA polymerase (Promega). PCR conditions were set at denaturation at 94°C for 1 min., annealing at 55°C for 1 min., and extension at 72°C for 2 min. for 29 cycles. A final cycle ended with 10 min. of extension at 72°C. Fragments were separated on 6% polyacrylamide denaturing gels. Dideoxy sequencing ladders of M13mpl8 DNA were used as fragment size standards. Other Comments: The human OMP sequence has been assigned EMBL accession number Z18943. Acknowledgements: Supported by NTH grants RO1DC00677, grant 89-198 from the Retinitis Pigmentosa Foundation, and the UK Human Genome Mapping Programme. References: 1) Brown.K.A. etal. (1992) Genomics 13, 189-193. 2) Evans.K.L. et al. (1993) Hum. Mol. Genet, in press.

PY - 1993/6

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Dinucleotide repeat polymorphism at the human olfactory marker protein (OMP) locus on chromosome 11q13.5 near tyrosinase (TYR)

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