Abstract
High-speed atomic force microscopy (HS-AFM) is an advanced technique with numerous applications in biology, particularly in molecular biophysics. Developed as a time-lapse AFM technique for direct imaging fully hydrated biological molecules, HS-AFM is currently capable of visualizing the dynamics of biological molecules and their complexes at a video-data acquisition rate. Spatial resolution at the nanometer level is another important characteristic of HS-AFM. This review focuses on examples of primarily protein-DNA complexes to illustrate the high temporal and spatial resolution capabilities of HS-AFM that have resulted in novel models and/or the functional mechanisms of these biological systems.
Original language | English (US) |
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Article number | 403001 |
Journal | Journal of Physics D: Applied Physics |
Volume | 51 |
Issue number | 40 |
DOIs | |
State | Published - Aug 20 2018 |
Keywords
- AFM
- chromatin dynamics
- nanoscale dynamics
- protein-DNA interactions
- time-lapse imaging
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Acoustics and Ultrasonics
- Surfaces, Coatings and Films