Direct analysis of RNA transcripts in electroporated carrot protoplasts

Elizabeth E. Murray; Wallace G. Buchholz; Benjamin Bowen

doi:10.1007/BF00232087

Direct analysis of RNA transcripts in electroporated carrot protoplasts

Elizabeth E. Murray, Wallace G. Buchholz, Benjamin Bowen

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We describe a method for direct analysis of RNA transcribed from DNA introduced into carrot cells by electroporation. Octopine synthase RNA transcribed from the plasmid p35SOcs was detected in total and poly A⁺ RNA on Northern blots and in RNA protection assays. The highest level of octopine synthase transcript was detected at approximately 8 hrs post-electroporation, although RNA could still be detected after 48 hrs. This method allows detection of foreign gene expression in a plant system and bypasses the need for reporter genes.

Original language	English (US)
Pages (from-to)	129-132
Number of pages	4
Journal	Plant Cell Reports
Volume	9
Issue number	3
DOIs	https://doi.org/10.1007/BF00232087
State	Published - Jul 1990
Externally published	Yes

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/BF00232087

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title = "Direct analysis of RNA transcripts in electroporated carrot protoplasts",

abstract = "We describe a method for direct analysis of RNA transcribed from DNA introduced into carrot cells by electroporation. Octopine synthase RNA transcribed from the plasmid p35SOcs was detected in total and poly A+ RNA on Northern blots and in RNA protection assays. The highest level of octopine synthase transcript was detected at approximately 8 hrs post-electroporation, although RNA could still be detected after 48 hrs. This method allows detection of foreign gene expression in a plant system and bypasses the need for reporter genes.",

author = "Murray, {Elizabeth E.} and Buchholz, {Wallace G.} and Benjamin Bowen",

year = "1990",

month = jul,

doi = "10.1007/BF00232087",

language = "English (US)",

volume = "9",

pages = "129--132",

journal = "Plant Cell Reports",

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publisher = "Springer Verlag",

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T1 - Direct analysis of RNA transcripts in electroporated carrot protoplasts

AU - Murray, Elizabeth E.

AU - Buchholz, Wallace G.

AU - Bowen, Benjamin

PY - 1990/7

Y1 - 1990/7

N2 - We describe a method for direct analysis of RNA transcribed from DNA introduced into carrot cells by electroporation. Octopine synthase RNA transcribed from the plasmid p35SOcs was detected in total and poly A+ RNA on Northern blots and in RNA protection assays. The highest level of octopine synthase transcript was detected at approximately 8 hrs post-electroporation, although RNA could still be detected after 48 hrs. This method allows detection of foreign gene expression in a plant system and bypasses the need for reporter genes.

AB - We describe a method for direct analysis of RNA transcribed from DNA introduced into carrot cells by electroporation. Octopine synthase RNA transcribed from the plasmid p35SOcs was detected in total and poly A+ RNA on Northern blots and in RNA protection assays. The highest level of octopine synthase transcript was detected at approximately 8 hrs post-electroporation, although RNA could still be detected after 48 hrs. This method allows detection of foreign gene expression in a plant system and bypasses the need for reporter genes.

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JO - Plant Cell Reports

JF - Plant Cell Reports

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ER -

Direct analysis of RNA transcripts in electroporated carrot protoplasts

Abstract

ASJC Scopus subject areas

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