"Direct" detection and separation of DNA using nanoporous alumina filters

Ivan Vlassiouk, Alexey Krasnoslobodtsev, Sergei Smirnov, Markus Germann

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

The concept of using alumina nanoporous filters (AAO) modified with DNA for "label-free" detection and separation/purification of the target ss-DNA is demonstrated. The high surface density of DNA (4 × 10 12 cm -2) and high efficiency of hybridization (ca. 70%) in combination with increased effective surface area make this system very attractive for development of various ss-DNA (or RNA) detection methods. Moderate transparency of AAO in the UV and IR regions allows direct detection of DNA hybridization by optical and IR absorption. Close to the quantitative efficiency of binding the target ss-DNA from solution using a single pass through the modified filter is observed.

Original languageEnglish (US)
Pages (from-to)9913-9915
Number of pages3
JournalLangmuir
Volume20
Issue number23
DOIs
StatePublished - Nov 9 2004

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Fingerprint Dive into the research topics of '"Direct" detection and separation of DNA using nanoporous alumina filters'. Together they form a unique fingerprint.

  • Cite this