Direct detection of double-stranded DNA: Molecular methods and applications for DNA diagnostics

Indraneel Ghosh, Cliff I. Stains, Aik T. Ooi, David J. Segal

Research output: Contribution to journalReview articlepeer-review

82 Scopus citations

Abstract

Methodologies to detect DNA sequences with high sensitivity and specificity have tremendous potential as molecular diagnostic agents. Most current methods exploit the ability of single-stranded DNA (ssDNA) to base pair with high specificity to a complementary molecule. However, recent advances in robust techniques for recognition of DNA in the major and minor groove have made possible the direct detection of double-stranded DNA (dsDNA), without the need for denaturation, renaturation, or hybridization. This review will describe the progress in adapting polyamides, triplex DNA, and engineered zinc finger DNA-binding proteins as dsDNA diagnostic systems. In particular, the sequence-enabled reassembly (SEER) method, involving the use of custom zinc finger proteins, offers the potential for direct detection of dsDNA in cells, with implications for cell-based diagnostics and therapeutics.

Original languageEnglish (US)
Pages (from-to)551-560
Number of pages10
JournalMolecular BioSystems
Volume2
Issue number11
DOIs
StatePublished - 2006
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Direct detection of double-stranded DNA: Molecular methods and applications for DNA diagnostics'. Together they form a unique fingerprint.

Cite this