Abstract
The tumor-promoting agent, 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibits the humoral immune response of lymphocytes to antigen. To test the hypothesis that this inhibition is due to a direct effect upon B lymphocytes, splenic lymphocytes or murine B lymphocytes, enriched by 'panning' splenic lymphocytes onto anti-IgM-coated petri dishes, were immunized in vitro with the thymus/accessory cell-independent antigen trinitrophenyl lipopolysaccharide (TNP-LPS) with or without TPA. The number of anti-TNP antibody-forming cells present in both lymphocyte populations after 5 days was almost completely inhibited to the same degree by TPA. These data unambiguously show that TPA can directly inhibit the differentiation of B lymphocytes to antibody-forming cells.
Original language | English (US) |
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Pages (from-to) | 157-163 |
Number of pages | 7 |
Journal | Cancer Letters |
Volume | 34 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1987 |
Externally published | Yes |
ASJC Scopus subject areas
- Oncology
- Cancer Research