TY - JOUR
T1 - Direct presentation of nonpeptide prenyl pyrophosphate antigens to human γδ T cells
AU - Morita, Craig T.
AU - Beckman, Evan M.
AU - Bukowski, Jack F.
AU - Tanaka, Yoshimasa
AU - Band, Hamid
AU - Bloom, Barry R.
AU - Golan, David E.
AU - Brenner, Michael B.
N1 - Funding Information:
Correspondence should be addressed to C. T. M. We thank C. Parker and S. Porcelli for critical reading of the manuscript. We thank Drs. D. Pious, R. DeMars, R. Accolla, and B. Lisowsk-Grospierre for providing mutant tumor cell lines. We thank Drs. J. IaSalle, D. Hafler. and M. Roncarolo for use of up T cell clones. We thank P. Lopez and P. Yacano for expert technical assistance. Thii work was supported by grants from the National Institutes of Health to M. 8. B. (CA47724). H. 8. (Al28508, AR36308) and to D. E. G. (HL32854, HL15157) and from the Howard Hughes Medical Institute (to B. R. B.). C. T. M. was supported by a fellowship from the Arthritis Foundation and M. B. B. is a Scholar of the Leukemia Society.
PY - 1995/10
Y1 - 1995/10
N2 - Human Vγ2Vδ2+ T cells recognize mycobacterial non-peptide antigens, such as isopentenyl pyrophosphate, and their synthetic analogs, such as monoethyl phosphate, through a TCR-dependent process. Here, we examine the presentation of these antigens. Vγ2Vδ2+ T cells recognized secreted prenyl pyrophosphate antigens in the absence of other accessory cells but, under such conditions, required T cell-T cell contact. Recognition required neither the expression of classical MHC class I, MHC class II, or CD1a, CD1b, and CD1c molecules, nor MHC class I or class II peptide loading pathways. Fixed accessory cells also presented the prenyl pyrophosphate antigens to γδ T cells. Thus, in contrast with the presentation of conventional peptide antigens, protein antigens, and superantigens to αβ T cells, prenyl pyrophosphate antigens are presented to γδ T cells through a novel extracellular pathway that does not require antigen uptake, antigen processing, or MHC class I or class II expression. This pathway allows for the rapid recognition of bacteria by γδ T cells and suggests that γδ T cells play a role in the early response to bacterial infection.
AB - Human Vγ2Vδ2+ T cells recognize mycobacterial non-peptide antigens, such as isopentenyl pyrophosphate, and their synthetic analogs, such as monoethyl phosphate, through a TCR-dependent process. Here, we examine the presentation of these antigens. Vγ2Vδ2+ T cells recognized secreted prenyl pyrophosphate antigens in the absence of other accessory cells but, under such conditions, required T cell-T cell contact. Recognition required neither the expression of classical MHC class I, MHC class II, or CD1a, CD1b, and CD1c molecules, nor MHC class I or class II peptide loading pathways. Fixed accessory cells also presented the prenyl pyrophosphate antigens to γδ T cells. Thus, in contrast with the presentation of conventional peptide antigens, protein antigens, and superantigens to αβ T cells, prenyl pyrophosphate antigens are presented to γδ T cells through a novel extracellular pathway that does not require antigen uptake, antigen processing, or MHC class I or class II expression. This pathway allows for the rapid recognition of bacteria by γδ T cells and suggests that γδ T cells play a role in the early response to bacterial infection.
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U2 - 10.1016/1074-7613(95)90178-7
DO - 10.1016/1074-7613(95)90178-7
M3 - Article
C2 - 7584140
AN - SCOPUS:0028829424
SN - 1074-7613
VL - 3
SP - 495
EP - 507
JO - Immunity
JF - Immunity
IS - 4
ER -