Direct processing of alginate-immobilized microalgae into polyhydroxybutyrate using marine bacterium of Saccharophagus degradans

Xinjuan Hu, Yulie E. Meneses, Jayne Stratton, Shuhao Huo

Research output: Contribution to journalArticlepeer-review

Abstract

Alginate immobilized microalgae (AIM) was found efficient in algal cells separation and pollutants removal, however, its processing required alginate removal. In present study, polysaccharide-degrading bacterium of Saccharophagus degradans was used to biodegrade alginate and microalgae in AIM and produce polyhydroxybutyrate (PHB). Results showed that AIM cultivated in wastewater contained 34.0% carbohydrate and 45.7% protein. S. degradans effectively degraded and utilized polysaccharide of AIM to maintain five-day continuous growth at 7.1–8.8 log CFU/mL. Compared with glucose, S. degradans metabolism of mixed polysaccharide in AIM maintained the medium pH at 7.1–7.8. Increasing the inoculum concentration did not enhance AIM utilization by S. degradans due to the carbon catabolite repression of glucose which likely inactivated hydrolysis enzymes. PHB production in S. degradans peaked at 64.9 mg/L after 72 h cultivation but was later degraded to provide energy. Conclusively, S. degradans was effective in direct processing of AIM while showing potential in PHB production.

Original languageEnglish (US)
Article number126898
JournalBioresource Technology
Volume351
DOIs
StatePublished - May 2022

Keywords

  • Alginate immobilization
  • Bioplastic
  • Microalgae
  • Polyhydroxyalkanoate
  • Saccharophagus degradans

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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