Discoidin domain receptor 2 is a critical regulator of epithelial-mesenchymal transition

Logan A. Walsh, Ali Nawshad, Damian Medici

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Discoidin domain receptor 2 (DDR2) is a collagen receptor that is expressed during epithelial-mesenchymal transition (EMT), a cellular transformation that mediates many stages of embryonic development and disease. However, the functional significance of this receptor in EMT is unknown. Here we show that Transforming Growth Factor-beta1 (TGF-β1), a common stimulator of EMT, promotes increased expression of type I collagen and DDR2. Inhibiting expression of COL1A1 or DDR2 with siRNA is sufficient to perturb activity of the NF-κB and LEF-1 transcription factors and to inhibit EMT and cell migration induced by TGF-β1. Furthermore, knockdown of DDR2 expression with siRNA inhibits EMT directly induced by type I collagen. These data establish a critical role for type I collagen-dependent DDR2 signaling in the regulation of EMT.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalMatrix Biology
Volume30
Issue number4
DOIs
StatePublished - May 2011

Keywords

  • Collagen
  • DDR2
  • EMT
  • Epithelial-mesenchymal transition
  • LEF-1
  • TGF-beta

ASJC Scopus subject areas

  • Molecular Biology

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