Abstract
Discoidin domain receptor 2 (DDR2) is a collagen receptor that is expressed during epithelial-mesenchymal transition (EMT), a cellular transformation that mediates many stages of embryonic development and disease. However, the functional significance of this receptor in EMT is unknown. Here we show that Transforming Growth Factor-beta1 (TGF-β1), a common stimulator of EMT, promotes increased expression of type I collagen and DDR2. Inhibiting expression of COL1A1 or DDR2 with siRNA is sufficient to perturb activity of the NF-κB and LEF-1 transcription factors and to inhibit EMT and cell migration induced by TGF-β1. Furthermore, knockdown of DDR2 expression with siRNA inhibits EMT directly induced by type I collagen. These data establish a critical role for type I collagen-dependent DDR2 signaling in the regulation of EMT.
Original language | English (US) |
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Pages (from-to) | 243-247 |
Number of pages | 5 |
Journal | Matrix Biology |
Volume | 30 |
Issue number | 4 |
DOIs | |
State | Published - May 2011 |
Keywords
- Collagen
- DDR2
- EMT
- Epithelial-mesenchymal transition
- LEF-1
- TGF-beta
ASJC Scopus subject areas
- Molecular Biology