@article{33346d6d590d4bdcba2b9d14fdecbfc4,
title = "Disruption of plastid acyl:acyl carrier protein synthetases increases medium chain fatty acid accumulation in seeds of transgenic Arabidopsis",
abstract = "Engineering transgenic plants that accumulate high levels of medium-chain fatty acids (MCFA) has been least successful for shorter chain lengths (e.g.; C8). We demonstrate that one limitation is the activity of acyl-ACP synthetase (AAE) that re-activates fatty acids released by acyl-ACP thioesterases. Seed expression of Cuphea pulcherrima FATB acyl-ACP thioesterase in a double mutant lacking AAE15/16 increased 8:0 accumulation almost 2-fold compared to expression in wild type. These results also provide an in planta demonstration that AAE enzymes participate not only in activation of exogenously added MCFA but also in activation of MCFA synthesized in plastids.",
keywords = "AAE15/16, Acyl chain activating enzyme, Cuphea, Medium chain fatty acid, Thioesterase, Triacylglycerol",
author = "Henrik Tjellstr{\"o}m and Merissa Strawsine and Jillian Silva and Cahoon, {Edgar B.} and Ohlrogge, {John B.}",
note = "Funding Information: This work was supported by the Great Lakes Bioenergy Research Center through the U.S. Department of Energy (Cooperative Agreement no. DE-FC02-07ER64494), the U.S. National Science Foundation (Grant No. DBI-0701919) to John B. Ohlrogge; Stiftelsen Olle Engkvist Byggm{\"a}stare and the Swedish Council for Environment, Agricultural Sciences and Spatial Planning (Project No. 2009-664) to Henrik Tjellstr{\"o}m. Research in the Cahoon lab was funded by the Center for Advanced Biofuel Systems (CABS), an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Basic Energy Sciences under Award Number DE-SC0001295. ESI-MS analyses were carried out in the Mass Spectrometry Facility at Michigan State University. ",
year = "2013",
month = apr,
day = "2",
doi = "10.1016/j.febslet.2013.02.021",
language = "English (US)",
volume = "587",
pages = "936--942",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "7",
}