@article{6aa4c88705084bd4bf7c262d0bc8aa99,
title = "Dissolvable Microneedles Coupled with Nanofiber Dressings Eradicate Biofilms via Effectively Delivering a Database-Designed Antimicrobial Peptide",
abstract = "Biofilms in chronic wounds, including diabetic foot ulcers, pressure ulcers, and venous leg ulcers, pose a major challenge to wound management. Herein, we report a Janus-type antimicrobial dressing for eradication of biofilms in chronic wounds. The dressing consists of electrospun nanofiber membranes coupled with dissolvable microneedle arrays to enable effective delivery of a database-designed antimicrobial peptide to both inside and outside biofilms. This antimicrobial dressing exhibited high efficacy against a broad spectrum of resistant pathogens in vitro. Importantly, such a dressing was able to eradicate methicillin-resistant Staphylococcus aureus (MRSA) biofilms in both an ex vivo human skin wound infection model and a type II diabetic mouse wound infection model after daily treatment without applying surgical debridement. Most importantly, the dressing can also completely remove the Pseudomonas aeruginosa and MRSA, dual-species biofilm in an ex vivo human skin infection model. In addition, our computational simulations also suggested that microneedles were more effective in the delivery of peptides to the biofilms than free drugs. Our results indicate that the Janus-type antimicrobial dressings may provide an effective treatment and management of chronic wound polymicrobial infections.",
keywords = "Janus-type dressing, antimicrobial peptide, biofilm, chronic wound, microneedle, nanofiber",
author = "Yajuan Su and Mainardi, {Valerio Luca} and Hongjun Wang and Alec McCarthy and Zhang, {Yu Shrike} and Shixuan Chen and John, {Johnson V.} and Wong, {Shannon L.} and Hollins, {Ronald R.} and Guangshun Wang and Jingwei Xie",
note = "Funding Information: This work was partially supported by startup funds from University of Nebraska Medical Center (UNMC), UNMC Regenerative Medicine Program pilot grant, NE LB606, National Institute of General Medical Science (NIGMS) of the National Institutes of Health (NIH) under Award Number R01GM123081 to J.X., 1R01GM138552 to J.X. and G.W. NIH grant R01AI105147 and Nebraska Research Initiative POC award to G.W., and NIH grants R21EB026175, R21EB025270, R00CA201603, R01EB028143 and R01GM134036, and the support by the Brigham Research Institute to Y.S.Z. We thank Qianhui Wu for help cultivating the bacteria. Funding Information: This work was partially supported by startup funds from University of Nebraska Medical Center (UNMC), UNMC Regenerative Medicine Program pilot grant, NE LB606, National Institute of General Medical Science (NIGMS) of the National Institutes of Health (NIH) under Award Number R01GM123081 to J.X., 1R01GM138552 to J.X. and G.W., NIH grant R01AI105147 and Nebraska Research Initiative POC award to G.W., and NIH grants R21EB026175, R21EB025270, R00CA201603, R01EB028143, and R01GM134036, and the support by the Brigham Research Institute to Y.S.Z. We thank Qianhui Wu for help cultivating the bacteria. Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",
year = "2020",
month = sep,
day = "22",
doi = "10.1021/acsnano.0c04527",
language = "English (US)",
volume = "14",
pages = "11775--11786",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "9",
}