TY - JOUR
T1 - Distinct subtypes of proprioceptive dorsal root ganglion neurons regulate adaptive proprioception in mice
AU - Wu, Haohao
AU - Petitpré, Charles
AU - Fontanet, Paula
AU - Sharma, Anil
AU - Bellardita, Carmelo
AU - Quadros, Rolen M.
AU - Jannig, Paulo R.
AU - Wang, Yiqiao
AU - Heimel, J. Alexander
AU - Cheung, Kylie K.Y.
AU - Wanderoy, Simone
AU - Xuan, Yang
AU - Meletis, Konstantinos
AU - Ruas, Jorge
AU - Gurumurthy, Channabasavaiah B.
AU - Kiehn, Ole
AU - Hadjab, Saida
AU - Lallemend, François
N1 - Funding Information:
We thank Prof. Fan Wang and Dr. Joriene de Nooij for the RGT and Egr3WGA mouse lines, respectively, the CLICK imaging Facility supported by the Knut and Alice Wal-lenberg Foundation, the Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX) for providing data storage resources, and Kuisong Song for technical implication in some results of Fig. 5j. We are also grateful to Prof. Sten Grillner for critical reading of the manuscript. This work was supported by grants from: Strat-Neuro, the Swedish Brain Foundation, Karolinska Institutet, the Swedish Research Council, KID funding (F.L. and S.H.); the Knut and Alice Wallenbergs Foundation (Wallenberg Academy Fellow), Ragnar Söderberg Foundation (Ragnar Söderberg Fellow in Medicine) and Ming Wai Lau Foundation (F.L.). F.L. is a Wallenberg Academy Fellow in Medicine and a MWLC investigator.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Proprioceptive neurons (PNs) are essential for the proper execution of all our movements by providing muscle sensory feedback to the central motor network. Here, using deep single cell RNAseq of adult PNs coupled with virus and genetic tracings, we molecularly identify three main types of PNs (Ia, Ib and II) and find that they segregate into eight distinct subgroups. Our data unveil a highly sophisticated organization of PNs into discrete sensory input channels with distinct spatial distribution, innervation patterns and molecular profiles. Altogether, these features contribute to finely regulate proprioception during complex motor behavior. Moreover, while Ib- and II-PN subtypes are specified around birth, Ia-PN subtypes diversify later in life along with increased motor activity. We also show Ia-PNs plasticity following exercise training, suggesting Ia-PNs are important players in adaptive proprioceptive function in adult mice.
AB - Proprioceptive neurons (PNs) are essential for the proper execution of all our movements by providing muscle sensory feedback to the central motor network. Here, using deep single cell RNAseq of adult PNs coupled with virus and genetic tracings, we molecularly identify three main types of PNs (Ia, Ib and II) and find that they segregate into eight distinct subgroups. Our data unveil a highly sophisticated organization of PNs into discrete sensory input channels with distinct spatial distribution, innervation patterns and molecular profiles. Altogether, these features contribute to finely regulate proprioception during complex motor behavior. Moreover, while Ib- and II-PN subtypes are specified around birth, Ia-PN subtypes diversify later in life along with increased motor activity. We also show Ia-PNs plasticity following exercise training, suggesting Ia-PNs are important players in adaptive proprioceptive function in adult mice.
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U2 - 10.1038/s41467-021-21173-9
DO - 10.1038/s41467-021-21173-9
M3 - Article
C2 - 33589589
AN - SCOPUS:85101451990
SN - 2041-1723
VL - 12
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 1026
ER -