Distribution of polyadenylated RNA sequences in the cytoplasm of Drosophila cells

W. Beatriz Levy, Angie Rizzino

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Complementary DNA (cDNA) was synthesized by reverse transcriptase, on a template of total cytoplasmic polyadenylated RNA from Drosophila cells. This cDNA was hybridized to an excess of total cytoplasmic polyadenylated RNA, polysomal polyadenylated RNA and non-polysomal cytoplasmic polyadenylated RNA. Our results show that a significant fraction of the total cytoplasmic polyadenylated RNA sequences are not bound to polysomes. However, the majority of these sequences are present in the population of non-polysomal polyadenylated cytoplasmic RNA molecules.

Original languageEnglish (US)
Pages (from-to)377-380
Number of pages4
JournalExperimental Cell Research
Volume106
Issue number2
DOIs
StatePublished - May 1977

ASJC Scopus subject areas

  • Cell Biology

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