Distribution of polyadenylated RNA sequences in the cytoplasm of Drosophila cells

W. Beatriz Levy; Angie Rizzino

doi:10.1016/0014-4827(77)90183-5

Distribution of polyadenylated RNA sequences in the cytoplasm of Drosophila cells

W. Beatriz Levy, Angie Rizzino

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Complementary DNA (cDNA) was synthesized by reverse transcriptase, on a template of total cytoplasmic polyadenylated RNA from Drosophila cells. This cDNA was hybridized to an excess of total cytoplasmic polyadenylated RNA, polysomal polyadenylated RNA and non-polysomal cytoplasmic polyadenylated RNA. Our results show that a significant fraction of the total cytoplasmic polyadenylated RNA sequences are not bound to polysomes. However, the majority of these sequences are present in the population of non-polysomal polyadenylated cytoplasmic RNA molecules.

Original language	English (US)
Pages (from-to)	377-380
Number of pages	4
Journal	Experimental Cell Research
Volume	106
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(77)90183-5
State	Published - May 1977
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(77)90183-5

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title = "Distribution of polyadenylated RNA sequences in the cytoplasm of Drosophila cells",

abstract = "Complementary DNA (cDNA) was synthesized by reverse transcriptase, on a template of total cytoplasmic polyadenylated RNA from Drosophila cells. This cDNA was hybridized to an excess of total cytoplasmic polyadenylated RNA, polysomal polyadenylated RNA and non-polysomal cytoplasmic polyadenylated RNA. Our results show that a significant fraction of the total cytoplasmic polyadenylated RNA sequences are not bound to polysomes. However, the majority of these sequences are present in the population of non-polysomal polyadenylated cytoplasmic RNA molecules.",

author = "{Beatriz Levy}, W. and Angie Rizzino",

note = "Funding Information: This work was supported in part by a NIH Fellowship (CA 01060) from NC1 to A. Rizzino.",

year = "1977",

month = may,

doi = "10.1016/0014-4827(77)90183-5",

language = "English (US)",

volume = "106",

pages = "377--380",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

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T1 - Distribution of polyadenylated RNA sequences in the cytoplasm of Drosophila cells

AU - Beatriz Levy, W.

AU - Rizzino, Angie

N1 - Funding Information: This work was supported in part by a NIH Fellowship (CA 01060) from NC1 to A. Rizzino.

PY - 1977/5

Y1 - 1977/5

N2 - Complementary DNA (cDNA) was synthesized by reverse transcriptase, on a template of total cytoplasmic polyadenylated RNA from Drosophila cells. This cDNA was hybridized to an excess of total cytoplasmic polyadenylated RNA, polysomal polyadenylated RNA and non-polysomal cytoplasmic polyadenylated RNA. Our results show that a significant fraction of the total cytoplasmic polyadenylated RNA sequences are not bound to polysomes. However, the majority of these sequences are present in the population of non-polysomal polyadenylated cytoplasmic RNA molecules.

AB - Complementary DNA (cDNA) was synthesized by reverse transcriptase, on a template of total cytoplasmic polyadenylated RNA from Drosophila cells. This cDNA was hybridized to an excess of total cytoplasmic polyadenylated RNA, polysomal polyadenylated RNA and non-polysomal cytoplasmic polyadenylated RNA. Our results show that a significant fraction of the total cytoplasmic polyadenylated RNA sequences are not bound to polysomes. However, the majority of these sequences are present in the population of non-polysomal polyadenylated cytoplasmic RNA molecules.

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U2 - 10.1016/0014-4827(77)90183-5

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SP - 377

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JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Distribution of polyadenylated RNA sequences in the cytoplasm of Drosophila cells

Abstract

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