TY - JOUR
T1 - Distribution of prosaposin in the rat nervous system
AU - Hosoda, Yoshiki
AU - Miyawaki, Kyojy
AU - Saito, Shouichiro
AU - Chen, Jie
AU - Bing, Xue
AU - Terashita, Takehiro
AU - Kobayashi, Naoto
AU - Araki, Nobukazu
AU - Shimokawa, Tetsuya
AU - Hamada, Fumihiko
AU - Sano, Akira
AU - Tanabe, Hirotaka
AU - Matsuda, Seiji
N1 - Funding Information:
Funding: This study was supported by the Ehime University INCS and in part by grants-in-aid for Scientific Research to S.M. (Exploratory Res. 19659380) from the Japan Society for the Promotion of Science and to AS (Priority Areas 18023029) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2007/11
Y1 - 2007/11
N2 - Prosaposin is the precursor of four sphingolipid activator proteins (saposins A, B, C, and D) for lysosomal hydrolases and is abundant in the nervous system and muscle. In addition to its role as a precursor of saposins in lysosomes, intact prosaposin has neurotrophic effects in vivo or in vitro when supplied exogenously. We examined the distribution of prosaposin in the central and peripheral nervous systems and its intracellular distribution. Using a monospecific antisaposin D antibody that crossreacts with prosaposin but not with saposins A, B, or C, immunoblot experiments showed that both the central and peripheral nervous systems express unprocessed prosaposin and little saposin D. Using the antisaposin D antibodies, we demonstrated that prosaposin is abundant in almost all neurons of both the central and peripheral nervous systems, including autonomic nerves, as well as motor and sensory nerves. Immunoelectron microscopy using double staining with antisaposin D and anticathepsin D antibodies showed strong prosaposin immunoreactivity mainly in the lysosomal granules in the neurons in both the central and peripheral nervous systems. The expression of prosaposin mRNA, examined using in situ hybridization, was observed in these same neurons. Our results suggest that prosaposin is synthesized ubiquitously in neurons of both the central and peripheral nervous systems.
AB - Prosaposin is the precursor of four sphingolipid activator proteins (saposins A, B, C, and D) for lysosomal hydrolases and is abundant in the nervous system and muscle. In addition to its role as a precursor of saposins in lysosomes, intact prosaposin has neurotrophic effects in vivo or in vitro when supplied exogenously. We examined the distribution of prosaposin in the central and peripheral nervous systems and its intracellular distribution. Using a monospecific antisaposin D antibody that crossreacts with prosaposin but not with saposins A, B, or C, immunoblot experiments showed that both the central and peripheral nervous systems express unprocessed prosaposin and little saposin D. Using the antisaposin D antibodies, we demonstrated that prosaposin is abundant in almost all neurons of both the central and peripheral nervous systems, including autonomic nerves, as well as motor and sensory nerves. Immunoelectron microscopy using double staining with antisaposin D and anticathepsin D antibodies showed strong prosaposin immunoreactivity mainly in the lysosomal granules in the neurons in both the central and peripheral nervous systems. The expression of prosaposin mRNA, examined using in situ hybridization, was observed in these same neurons. Our results suggest that prosaposin is synthesized ubiquitously in neurons of both the central and peripheral nervous systems.
KW - Immunohistochemistry
KW - In situ hybridization
KW - Nervous system
KW - Neurotrophic factor
KW - Prosaposin
KW - Rat (Wistar)
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U2 - 10.1007/s00441-007-0464-9
DO - 10.1007/s00441-007-0464-9
M3 - Article
C2 - 17763872
AN - SCOPUS:34848856743
SN - 0302-766X
VL - 330
SP - 197
EP - 207
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 2
ER -