DNA-gold nanoprobe-based integrated biosensing technology for non-invasive liquid biopsy of serum miRNA: A new frontier in prostate cancer diagnosis

Prakash Kshirsagar, Parthasarathy Seshacharyulu, Sakthivel Muniyan, Satyanarayan Rachagani, Lynette M. Smith, Christopher Thompson, Ashu Shah, Kavita Mallya, Sushil Kumar, Maneesh Jain, Surinder K. Batra

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The low specificity of prostate-specific antigen contributes to overdiagnosis and ov ertreatment of prostate cancer (PCa) patients. Hence, there is an urgent need for inclusive diagnostic platforms that could improve the diagnostic accuracy of PCa. Dysregulated miRNAs are closely associated with the progression and recurrence and have emerged as promising diagnostic and prognostic biomarkers for PCa. Nevertheless, simple, rapid, and ultrasensitive quantification of serum miRNAs is highly challenging. This study designed, synthesized, and demonstrated the practicability of DNA-linked gold nanoprobes (DNA-AuNPs) for the single-step quantification of miR-21/miR-141/miR-375. In preclinical study, the assay differented PCa Pten conditional knockout (PtencKO) mice compared to their age-matched Pten wild-type (PtenWT) control mice. In human sera, receiver operating characteristic (ROC) curve-based correlation analyses revealed clear discrimination between PCa patients from normal healthy controls using training and validation sets. Overall, we established integrated nano-biosensing technology for the PCR-free, non-invasive liquid biopsies of multiple miRNAs for PCa diagnosis.

Original languageEnglish (US)
Article number102566
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume43
DOIs
StatePublished - Jul 2022

Keywords

  • DSN
  • Fluorescence
  • Gold nanoprobes
  • Liquid biopsy
  • Non-invasive diagnosis
  • PSA
  • Prostate cancer
  • Serum miRNA

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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