DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga

James L. van Etten; Anne M. Schuster; Lois Girton; Dwight E. Burbank; David Swinton; Stanley Hattman

doi:10.1093/nar/13.10.3471

DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga

James L. van Etten, Anne M. Schuster, Lois Girton, Dwight E. Burbank, David Swinton, Stanley Hattman

Plant Pathology

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

The genomic DNAs of the eukaryotic Chlorella-like green alga, strain NC64A, and eleven of its viruses all contain significant levels of 5-methyldeoxycytidine. In addition, the host DNA as well as six of the viral DNAs also contain N⁶-methyldeoxyadenosine. At least some of the methylated bases in the host reside in different base sequences than the nethylated bases in the viruses as shown by differential susceptibility to restriction endonuclease enzymes. This suggests that the viruses encode for DNA methyltransferases with sequence specificities different from that of the host enzyme.

Original language	English (US)
Pages (from-to)	3471-3478
Number of pages	8
Journal	Nucleic acids research
Volume	13
Issue number	10
DOIs	https://doi.org/10.1093/nar/13.10.3471
State	Published - May 24 1985

ASJC Scopus subject areas

Genetics

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title = "DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga",

abstract = "The genomic DNAs of the eukaryotic Chlorella-like green alga, strain NC64A, and eleven of its viruses all contain significant levels of 5-methyldeoxycytidine. In addition, the host DNA as well as six of the viral DNAs also contain N6-methyldeoxyadenosine. At least some of the methylated bases in the host reside in different base sequences than the nethylated bases in the viruses as shown by differential susceptibility to restriction endonuclease enzymes. This suggests that the viruses encode for DNA methyltransferases with sequence specificities different from that of the host enzyme.",

author = "{van Etten}, {James L.} and Schuster, {Anne M.} and Lois Girton and Burbank, {Dwight E.} and David Swinton and Stanley Hattman",

note = "Funding Information: ACKNOWLEDGMENTS We thank M. G. Narinus for kindly providing fi. coli strain GH2163. The helpful discussions with Myron Brakke, Les Lane, and Rues Heints are gratefully acknowledged. This investigation was supported, in part, by Public Health Service grants Gm-32441 (JVE) and GM-29227 (SH) from the National Institute of General Medical Sciences and grant DE-AC02-82ER12086 (JVE) from the Department of Energy.",

year = "1985",

month = may,

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T1 - DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga

AU - van Etten, James L.

AU - Schuster, Anne M.

AU - Girton, Lois

AU - Burbank, Dwight E.

AU - Swinton, David

AU - Hattman, Stanley

N1 - Funding Information: ACKNOWLEDGMENTS We thank M. G. Narinus for kindly providing fi. coli strain GH2163. The helpful discussions with Myron Brakke, Les Lane, and Rues Heints are gratefully acknowledged. This investigation was supported, in part, by Public Health Service grants Gm-32441 (JVE) and GM-29227 (SH) from the National Institute of General Medical Sciences and grant DE-AC02-82ER12086 (JVE) from the Department of Energy.

PY - 1985/5/24

Y1 - 1985/5/24

N2 - The genomic DNAs of the eukaryotic Chlorella-like green alga, strain NC64A, and eleven of its viruses all contain significant levels of 5-methyldeoxycytidine. In addition, the host DNA as well as six of the viral DNAs also contain N6-methyldeoxyadenosine. At least some of the methylated bases in the host reside in different base sequences than the nethylated bases in the viruses as shown by differential susceptibility to restriction endonuclease enzymes. This suggests that the viruses encode for DNA methyltransferases with sequence specificities different from that of the host enzyme.

AB - The genomic DNAs of the eukaryotic Chlorella-like green alga, strain NC64A, and eleven of its viruses all contain significant levels of 5-methyldeoxycytidine. In addition, the host DNA as well as six of the viral DNAs also contain N6-methyldeoxyadenosine. At least some of the methylated bases in the host reside in different base sequences than the nethylated bases in the viruses as shown by differential susceptibility to restriction endonuclease enzymes. This suggests that the viruses encode for DNA methyltransferases with sequence specificities different from that of the host enzyme.

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