DNA polymerase chain reaction: A model of error frequencies and extension rates

Mark Griep, Scott Whitney, Michael Nelson, Hendrik Viljoen

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The polymerase chain reaction (PCR) is one of the most important reactions in molecular biology. The detailed mechanistic studies of the polymerase chain reaction have revealed a complex sequence of reversible reactions that involve intermediaries and activated complexes. The DNA polymerase does not merely facilitate the insertion of dNMP (deoxynucleotide monophosphates), but it also performs rapid screening of substrates to ensure a high degree of fidelity. The main result of this study is an expression for the average extension rate of the enzyme. The model is versatile and additional complexities, such as the type of nucleotide to be inserted, the GC content of the sequence in the vicinity of the insertion site, and the topology of the template, such as kinks and hairpins, are easy to incorporate. The insertion of incorrect nucleotides into the sequence is also addressed. Expressions to predict error frequencies are presented. It is shown that a relation exists between error frequency and extension rate: the error frequency is a minimum when the extension rate is optimal.

Original languageEnglish (US)
Pages (from-to)384-392
Number of pages9
JournalAIChE Journal
Volume52
Issue number1
DOIs
StatePublished - Jan 1 2006

Keywords

  • Mathematical model
  • Molecular biology
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Chemical Engineering(all)

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