DNA polymorphism and molecular subtyping of the capsular gene cluster of group B Streptococcus

Shannon D. Manning; David W. Lacher; H. Dele Davies; Betsy Foxman; Thomas S. Whittam

doi:10.1128/JCM.43.12.6113-6116.2005

DNA polymorphism and molecular subtyping of the capsular gene cluster of group B Streptococcus

Shannon D. Manning, David W. Lacher, H. Dele Davies, Betsy Foxman, Thomas S. Whittam

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping.

Original language	English (US)
Pages (from-to)	6113-6116
Number of pages	4
Journal	Journal of clinical microbiology
Volume	43
Issue number	12
DOIs	https://doi.org/10.1128/JCM.43.12.6113-6116.2005
State	Published - Dec 2005
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.43.12.6113-6116.2005

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abstract = "Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping.",

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AU - Whittam, Thomas S.

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AB - Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping.

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DNA polymorphism and molecular subtyping of the capsular gene cluster of group B Streptococcus

Abstract

ASJC Scopus subject areas

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