DNA sequence-enabled reassembly of the green fluorescent protein

Cliff I. Stains, Jason R. Porter, Aik T. Ooi, David J. Segal, Indraneel Ghosh

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

We describe a general methodology for the direct detection of DNA by the design of a split-protein system that reassembles to form an active complex only in the presence of a targeted DNA sequence. This approach, called SEquence Enabled Reassembly (SEER) of proteins, combines the ability to rationally dissect proteins to construct oligomerization-dependent protein reassembly systems and the availability of DNA binding Cys2-His2 zinc-finger motifs for the recognition of specific DNA sequences. We demonstrate the feasibility of the SEER approach utilizing the split green fluorescent protein appended to appropriate zinc fingers, such that chromophore formation is only catalyzed in the presence of DNA sequences that incorporate binding sites for both zinc fingers.

Original languageEnglish (US)
Pages (from-to)10782-10783
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number31
DOIs
StatePublished - Aug 10 2005

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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