DNA synthesis in a Chlorella-like alga following infection with the virus PBCV-1

James L. Van Etten; Dwight E. Burbank; Jay Joshi; Russel H. Meints

doi:10.1016/0042-6822(84)90311-8

DNA synthesis in a Chlorella-like alga following infection with the virus PBCV-1

James L. Van Etten, Dwight E. Burbank, Jay Joshi, Russel H. Meints

Plant Pathology

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

Infection of the unicellular, eukaryotic Chlorella-like alga NC64A by the large dsDNA virus, PBCV-1, resulted in a threefold increase in total DNA by 4 hr post infection. Viral infection rapidly inhibited host DNA synthesis which was followed by the degradation of the host chloroplast and nuclear DNA. Viral DNA synthesis began 30 to 40 min after infection and was dependent on de novo protein synthesis. Thus, the virus does not carry all of the components required to form a functional viral DNA polymerase into the cell.

Original language	English (US)
Pages (from-to)	443-449
Number of pages	7
Journal	Virology
Volume	134
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(84)90311-8
State	Published - Apr 30 1984

ASJC Scopus subject areas

Virology

Access to Document

10.1016/0042-6822(84)90311-8

Cite this

@article{704852a118b84fb6854cd206b9fea335,

title = "DNA synthesis in a Chlorella-like alga following infection with the virus PBCV-1",

abstract = "Infection of the unicellular, eukaryotic Chlorella-like alga NC64A by the large dsDNA virus, PBCV-1, resulted in a threefold increase in total DNA by 4 hr post infection. Viral infection rapidly inhibited host DNA synthesis which was followed by the degradation of the host chloroplast and nuclear DNA. Viral DNA synthesis began 30 to 40 min after infection and was dependent on de novo protein synthesis. Thus, the virus does not carry all of the components required to form a functional viral DNA polymerase into the cell.",

author = "{Van Etten}, {James L.} and Burbank, {Dwight E.} and Jay Joshi and Meints, {Russel H.}",

note = "Funding Information: We thank Myron Brakke, Les Lane, George Veo-mett, and Bob Schmidt for helpful discussions and Lawrence Bogorad for providing plasmid pZmc100. This investigation was supported in part by Department of Energy Grant DE AC02-82ER12086.",

year = "1984",

month = apr,

day = "30",

doi = "10.1016/0042-6822(84)90311-8",

language = "English (US)",

volume = "134",

pages = "443--449",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - DNA synthesis in a Chlorella-like alga following infection with the virus PBCV-1

AU - Van Etten, James L.

AU - Burbank, Dwight E.

AU - Joshi, Jay

AU - Meints, Russel H.

N1 - Funding Information: We thank Myron Brakke, Les Lane, George Veo-mett, and Bob Schmidt for helpful discussions and Lawrence Bogorad for providing plasmid pZmc100. This investigation was supported in part by Department of Energy Grant DE AC02-82ER12086.

PY - 1984/4/30

Y1 - 1984/4/30

N2 - Infection of the unicellular, eukaryotic Chlorella-like alga NC64A by the large dsDNA virus, PBCV-1, resulted in a threefold increase in total DNA by 4 hr post infection. Viral infection rapidly inhibited host DNA synthesis which was followed by the degradation of the host chloroplast and nuclear DNA. Viral DNA synthesis began 30 to 40 min after infection and was dependent on de novo protein synthesis. Thus, the virus does not carry all of the components required to form a functional viral DNA polymerase into the cell.

AB - Infection of the unicellular, eukaryotic Chlorella-like alga NC64A by the large dsDNA virus, PBCV-1, resulted in a threefold increase in total DNA by 4 hr post infection. Viral infection rapidly inhibited host DNA synthesis which was followed by the degradation of the host chloroplast and nuclear DNA. Viral DNA synthesis began 30 to 40 min after infection and was dependent on de novo protein synthesis. Thus, the virus does not carry all of the components required to form a functional viral DNA polymerase into the cell.

UR - http://www.scopus.com/inward/record.url?scp=0021262414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021262414&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(84)90311-8

DO - 10.1016/0042-6822(84)90311-8

M3 - Article

C2 - 18639814

AN - SCOPUS:0021262414

SN - 0042-6822

VL - 134

SP - 443

EP - 449

JO - Virology

JF - Virology

IS - 2

ER -

DNA synthesis in a Chlorella-like alga following infection with the virus PBCV-1

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this