@article{de2a7c95a9a6427ead494100da8a8088,
title = "DNA-templated fluorescent silver nanoclusters inhibit bacterial growth while being non-toxic to mammalian cells",
abstract = "Silver has a long history of antibacterial effectiveness. The combination of atomically precise metal nanoclusters with the field of nucleic acid nanotechnology has given rise to DNA-templated silver nanoclusters (DNA-AgNCs) which can be engineered with reproducible and unique fluorescent properties and antibacterial activity. Furthermore, cytosine-rich single-stranded DNA oligonucleotides designed to fold into hairpin structures improve the stability of AgNCs and additionally modulate their antibacterial properties and the quality of observed fluorescent signals. In this work, we characterize the sequence-specific fluorescence and composition of four representative DNA-AgNCs, compare their corresponding antibacterial effectiveness at different pH, and assess cytotoxicity to several mammalian cell lines.",
keywords = "AgNC, Antibacterial, DNA, Fluorescence, Nanocluster, Silver",
author = "Lewis Rolband and Liam Yourston and Morgan Chandler and Damian Beasock and Leyla Danai and Seraphim Kozlov and Nolan Marshall and Oleg Shevchenko and Krasnoslobodtsev, {Alexey V.} and Afonin, {Kirill A.}",
note = "Funding Information: Research reported in this publication was supported by the National Institute Of General Medical Sciences of the National Institutes of Health under Award Number R35GM139587 (to K.A.A.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.The authors would like to thank Brittany Johnson (UNCC) for advice with bacterial studies, the Troutman Lab (UNCC) for use of the Molecular Devices SpectraMax M5 microplate reader for initial data collection, and Ryan Riskowski (UNO) for use of the Duetta Spectrometer. AFM scans were obtained with the use of MultiMode Nanoscope IV system at the Nanoimaging Core Facility, UNMC, and we also thank Alexander Lushnikov (NCF-UNMC) for his assistance in sample preparation for AFM imaging. Funding Information: Funding: Research reported in this publication was supported by the National Institute Of General Medical Sciences of the National Institutes of Health under Award Number R35GM139587 (to K.A.A.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = jul,
day = "1",
doi = "10.3390/molecules26134045",
language = "English (US)",
volume = "26",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "13",
}