DnaK-Mediated alterations in human growth hormone protein inclusion bodies

Paul Blum; Mark Velligan; Norman Lin; Abdul Matin

doi:10.1038/nbt0392-301

DnaK-Mediated alterations in human growth hormone protein inclusion bodies

Paul Blum, Mark Velligan, Norman Lin, Abdul Matin

Biological Sciences

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Cooverproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.

Original language	English (US)
Pages (from-to)	301-304
Number of pages	4
Journal	Bio/Technology
Volume	10
Issue number	3
DOIs	https://doi.org/10.1038/nbt0392-301
State	Published - Mar 1992

ASJC Scopus subject areas

Biotechnology

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title = "DnaK-Mediated alterations in human growth hormone protein inclusion bodies",

abstract = "Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Cooverproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.",

author = "Paul Blum and Mark Velligan and Norman Lin and Abdul Matin",

year = "1992",

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doi = "10.1038/nbt0392-301",

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AU - Blum, Paul

AU - Velligan, Mark

AU - Lin, Norman

AU - Matin, Abdul

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Y1 - 1992/3

N2 - Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Cooverproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.

AB - Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Cooverproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.

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DnaK-Mediated alterations in human growth hormone protein inclusion bodies

Abstract

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