Dominant-negative and knockdown approaches to studying PPAR activity

Sudhir Singh; Robert G. Bennett

doi:10.1007/978-1-62703-155-4_6

Dominant-negative and knockdown approaches to studying PPAR activity

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Manipulation of PPAR activity is often a valuable approach toward elucidation of the cellular effects of PPARs. The activity of specific PPARs can be decreased using chemical inhibitors, but these approaches can be affected by nonspecific interactions or cell toxicity. Alternative approaches include targeting PPAR gene expression or activity through molecular biology strategies. Here, we describe the targeting of PPARγ through dominant-negative and siRNA-mediated knockdown constructs.

Original language	English (US)
Title of host publication	Peroxisome Proliferator-Activated Receptors (PPARs)
Subtitle of host publication	Methods and Protocols
Editors	Mostafa Badr, Jihan Youssef
Pages	87-98
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-62703-155-4_6
State	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	952
ISSN (Print)	1064-3745

Keywords

Adenovirus
Dominant-negative
Peroxisome proliferator-activated receptor
siRNA

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-62703-155-4_6

Cite this

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AB - Manipulation of PPAR activity is often a valuable approach toward elucidation of the cellular effects of PPARs. The activity of specific PPARs can be decreased using chemical inhibitors, but these approaches can be affected by nonspecific interactions or cell toxicity. Alternative approaches include targeting PPAR gene expression or activity through molecular biology strategies. Here, we describe the targeting of PPARγ through dominant-negative and siRNA-mediated knockdown constructs.

KW - Adenovirus

KW - Dominant-negative

KW - Peroxisome proliferator-activated receptor

KW - siRNA

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ER -

Dominant-negative and knockdown approaches to studying PPAR activity

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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