Dominant-negative and knockdown approaches to studying PPAR activity

Sudhir Singh, Robert G. Bennett

Research output: Chapter in Book/Report/Conference proceedingChapter


Manipulation of PPAR activity is often a valuable approach toward elucidation of the cellular effects of PPARs. The activity of specific PPARs can be decreased using chemical inhibitors, but these approaches can be affected by nonspecific interactions or cell toxicity. Alternative approaches include targeting PPAR gene expression or activity through molecular biology strategies. Here, we describe the targeting of PPARγ through dominant-negative and siRNA-mediated knockdown constructs.

Original languageEnglish (US)
Title of host publicationPeroxisome Proliferator-Activated Receptors (PPARs)
Subtitle of host publicationMethods and Protocols
EditorsMostafa Badr, Jihan Youssef
Number of pages12
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Adenovirus
  • Dominant-negative
  • Peroxisome proliferator-activated receptor
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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