Double in situ hybridization for simultaneous detection of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus (PCV)

Theerapol Sirinarumitr, Steven D. Sorden, Igor Morozov, Prem S. Paul

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A double in situ hybridization method for the simultaneous detection of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus (PCV) genomes in the same tissue section was applied to lung tissues from 9 pigs in which PRRSV and PCV coinfection had been previously demonstrated. Paraffin-embedded tissue sections were simultaneously hybridized with a digoxigenin-labeled antisense RNA probe for PRRSV and a fluorescein-labeled antisense RNA probe for PCV, and hybridization was detected with anti-digoxigenin alkaline phosphatase/fast red and anti-fluorescein peroxidase/diaminobenzidine, respectively. PRRSV and PCV genomes were identified in the same pulmonary cell types as reported previously in all 9 pigs. In all pigs, PCV-positive cells outnumbered PRRSV-positive cells. A small proportion of alveolar macrophages contained both PRRSV and PCV genomes.

Original languageEnglish (US)
Pages (from-to)68-71
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume13
Issue number1
DOIs
StatePublished - Jan 2001

ASJC Scopus subject areas

  • veterinary(all)

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