TY - JOUR
T1 - Down-regulation of heme oxygenase-1 by hepatitis C virus infection in vivo and by the in vitro expression of hepatitis C core protein
AU - Abdalla, Maher Y.
AU - Britigan, Bradley E.
AU - Wen, Feng
AU - Icardi, Michael
AU - McCormick, Michael L.
AU - LaBrecque, Douglas R.
AU - Voigt, Michael
AU - Brown, Kyle E.
AU - Schmidt, Warren N.
N1 - Funding Information:
Financial support: Veterans Administration (Merit Review grants to B.E.B., M.L.M., and K.E.B.); National Institutes of Health (grants RO1 AA13215-01 and RO3 DK54842-03 to W.N.S. and RO1 AI34954 to B.E.B.); University of Iowa Carver Trust Foundation (to W.N.S. and B.E.B.).
PY - 2004/9/15
Y1 - 2004/9/15
N2 - Antioxidant enzymes, including heme oxygenase (HO)-1, are an important line of defense against oxidant-mediated liver injury. Because hepatitis C virus (HCV) infection appears to increase the production of oxidants, we evaluated levels of antioxidant enzymes and HO-1 in liver-biopsy samples from HCV-infected patients by immunoblot and semiquantitative reverse-transcriptase polymerase chain reaction. In HCV-infected liver samples, levels of immunoreactive HO-1 and HO-1 mRNA were >4-fold lower than levels in control samples, but levels of superoxide dismutase and catalase were unaffected. Immunohistochemical results confirmed the decreased expression of HO-1 in hepatocytes from liver samples from HCV-infected patients but not in those from patients with other chronic liver diseases. The expression of HO-1 was also reduced in cell lines that stably express HCV core protein, which suggests that core gene products are capable of regulating the expression of HO-1.
AB - Antioxidant enzymes, including heme oxygenase (HO)-1, are an important line of defense against oxidant-mediated liver injury. Because hepatitis C virus (HCV) infection appears to increase the production of oxidants, we evaluated levels of antioxidant enzymes and HO-1 in liver-biopsy samples from HCV-infected patients by immunoblot and semiquantitative reverse-transcriptase polymerase chain reaction. In HCV-infected liver samples, levels of immunoreactive HO-1 and HO-1 mRNA were >4-fold lower than levels in control samples, but levels of superoxide dismutase and catalase were unaffected. Immunohistochemical results confirmed the decreased expression of HO-1 in hepatocytes from liver samples from HCV-infected patients but not in those from patients with other chronic liver diseases. The expression of HO-1 was also reduced in cell lines that stably express HCV core protein, which suggests that core gene products are capable of regulating the expression of HO-1.
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U2 - 10.1086/423488
DO - 10.1086/423488
M3 - Article
C2 - 15319861
AN - SCOPUS:4444238755
SN - 0022-1899
VL - 190
SP - 1109
EP - 1118
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -