TY - JOUR
T1 - Downregulation of an evolutionary young mir-1290 in an ipscderived neural stem cell model of autism spectrum disorder
AU - Moore, Dalia
AU - Meays, Brittney M.
AU - Madduri, Lepakshe S.V.
AU - Shahjin, Farah
AU - Chand, Subhash
AU - Niu, Meng
AU - Albahrani, Abrar
AU - Guda, Chittibabu
AU - Pendyala, Gurudutt
AU - Fox, Howard S.
AU - Yelamanchili, Sowmya V.
N1 - Publisher Copyright:
Copyright © 2019 Dalia Moore et al.
PY - 2019
Y1 - 2019
N2 - The identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human-specific disease processes. We previously have identified an evolutionary young miRNA, miR-1290, to be essential in neural stem cell proliferation and neuronal differentiation. Here, we show that miR-1290 is significantly downregulated during neuronal differentiation in reprogrammed induced pluripotent stem cell-(iPSC-) derived neurons obtained from idiopathic autism spectrum disorder (ASD) patients. Further, we identified that miR-1290 is actively released into extracellular vesicles. Supplementing ASD patient-derived neural stem cells (NSCs) with conditioned media from differentiated control-NSCs spiked with "artificial EVs" containing synthetic miR-1290 oligonucleotides significantly rescued differentiation deficits in ASD cell lines. Based on our earlier published study and the observations from the data presented here, we conclude that miR-1290 regulation could play a critical role during neuronal differentiation in early brain development.
AB - The identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human-specific disease processes. We previously have identified an evolutionary young miRNA, miR-1290, to be essential in neural stem cell proliferation and neuronal differentiation. Here, we show that miR-1290 is significantly downregulated during neuronal differentiation in reprogrammed induced pluripotent stem cell-(iPSC-) derived neurons obtained from idiopathic autism spectrum disorder (ASD) patients. Further, we identified that miR-1290 is actively released into extracellular vesicles. Supplementing ASD patient-derived neural stem cells (NSCs) with conditioned media from differentiated control-NSCs spiked with "artificial EVs" containing synthetic miR-1290 oligonucleotides significantly rescued differentiation deficits in ASD cell lines. Based on our earlier published study and the observations from the data presented here, we conclude that miR-1290 regulation could play a critical role during neuronal differentiation in early brain development.
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U2 - 10.1155/2019/8710180
DO - 10.1155/2019/8710180
M3 - Article
C2 - 31191687
AN - SCOPUS:85071497716
SN - 1687-966X
VL - 2019
JO - Stem Cells International
JF - Stem Cells International
M1 - 8710180
ER -