TY - JOUR
T1 - Downregulation of an evolutionary young mir-1290 in an ipscderived neural stem cell model of autism spectrum disorder
AU - Moore, Dalia
AU - Meays, Brittney M.
AU - Madduri, Lepakshe S.V.
AU - Shahjin, Farah
AU - Chand, Subhash
AU - Niu, Meng
AU - Albahrani, Abrar
AU - Guda, Chittibabu
AU - Pendyala, Gurudutt
AU - Fox, Howard S.
AU - Yelamanchili, Sowmya V.
N1 - Funding Information:
We would like to thank Mr. Tom Bargar at the UNMC core electron microscopy facility for their assistance with the TEM analysis. We thank the Bioinformatics and Systems Biology Core at UNMC, which receives support from Nebraska Research Initiative (NRI) and NIH awards (2P20GM103427 and 5P30CA036727). This study was supported by Neurosensory COBRE-mentored pilot award P30GM110768 to S.Y.
Publisher Copyright:
Copyright © 2019 Dalia Moore et al.
PY - 2019
Y1 - 2019
N2 - The identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human-specific disease processes. We previously have identified an evolutionary young miRNA, miR-1290, to be essential in neural stem cell proliferation and neuronal differentiation. Here, we show that miR-1290 is significantly downregulated during neuronal differentiation in reprogrammed induced pluripotent stem cell-(iPSC-) derived neurons obtained from idiopathic autism spectrum disorder (ASD) patients. Further, we identified that miR-1290 is actively released into extracellular vesicles. Supplementing ASD patient-derived neural stem cells (NSCs) with conditioned media from differentiated control-NSCs spiked with "artificial EVs" containing synthetic miR-1290 oligonucleotides significantly rescued differentiation deficits in ASD cell lines. Based on our earlier published study and the observations from the data presented here, we conclude that miR-1290 regulation could play a critical role during neuronal differentiation in early brain development.
AB - The identification of several evolutionary young miRNAs, which arose in primates, raised several possibilities for the role of such miRNAs in human-specific disease processes. We previously have identified an evolutionary young miRNA, miR-1290, to be essential in neural stem cell proliferation and neuronal differentiation. Here, we show that miR-1290 is significantly downregulated during neuronal differentiation in reprogrammed induced pluripotent stem cell-(iPSC-) derived neurons obtained from idiopathic autism spectrum disorder (ASD) patients. Further, we identified that miR-1290 is actively released into extracellular vesicles. Supplementing ASD patient-derived neural stem cells (NSCs) with conditioned media from differentiated control-NSCs spiked with "artificial EVs" containing synthetic miR-1290 oligonucleotides significantly rescued differentiation deficits in ASD cell lines. Based on our earlier published study and the observations from the data presented here, we conclude that miR-1290 regulation could play a critical role during neuronal differentiation in early brain development.
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U2 - 10.1155/2019/8710180
DO - 10.1155/2019/8710180
M3 - Article
C2 - 31191687
AN - SCOPUS:85071497716
SN - 1687-9678
VL - 2019
JO - Stem Cells International
JF - Stem Cells International
M1 - 8710180
ER -