TY - JOUR
T1 - Dual-column determination of albumin and immunoglobulin G in serum by high-performance affinity chromatography
AU - Hage, David S.
AU - Walters, Rodney R.
N1 - Funding Information:
This work was supportedb y the National ScienceF oundation under Grant CHE-8305057.D .S.H. was supportedb y an AmericanC hemicalS ocietyA nalytical Division Fellowshipf rom the Proctor and Gamble Company.
PY - 1987
Y1 - 1987
N2 - High-performance affinity chromatography was used for the simultaneous determination of albumin and immunoglobulin G in serum. Two columns in series, the first containing immobilized anti-albumin antibodies and the other containing protein A for binding immunoglobulin G, were eluted separately at pH 3 by means of a column-switching system. This method gave results in good agreement with commercially available methods, while requiring only 2 μl of serum and 6.0 min per cycle. It was shown that albumin and immunoglobulin G were selectively retained, with little interference from other components, including immunoglobulins A and M.
AB - High-performance affinity chromatography was used for the simultaneous determination of albumin and immunoglobulin G in serum. Two columns in series, the first containing immobilized anti-albumin antibodies and the other containing protein A for binding immunoglobulin G, were eluted separately at pH 3 by means of a column-switching system. This method gave results in good agreement with commercially available methods, while requiring only 2 μl of serum and 6.0 min per cycle. It was shown that albumin and immunoglobulin G were selectively retained, with little interference from other components, including immunoglobulins A and M.
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U2 - 10.1016/S0021-9673(01)94582-0
DO - 10.1016/S0021-9673(01)94582-0
M3 - Article
C2 - 3104379
AN - SCOPUS:0023160445
VL - 386
SP - 37
EP - 49
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -