Abstract
High-performance affinity chromatography was used for the simultaneous determination of albumin and immunoglobulin G in serum. Two columns in series, the first containing immobilized anti-albumin antibodies and the other containing protein A for binding immunoglobulin G, were eluted separately at pH 3 by means of a column-switching system. This method gave results in good agreement with commercially available methods, while requiring only 2 μl of serum and 6.0 min per cycle. It was shown that albumin and immunoglobulin G were selectively retained, with little interference from other components, including immunoglobulins A and M.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 37-49 |
| Number of pages | 13 |
| Journal | Journal of Chromatography A |
| Volume | 386 |
| Issue number | C |
| DOIs | |
| State | Published - 1987 |
| Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry