TY - JOUR
T1 - Dynamic change of hydrogen sulfide during global cerebral ischemia-reperfusion and its effect in rats
AU - Ren, Caili
AU - Du, Ailin
AU - Li, Dongliang
AU - Sui, Jinwen
AU - Mayhan, William G.
AU - Zhao, Honggang
PY - 2010/7/23
Y1 - 2010/7/23
N2 - Hydrogen sulfide (H2S) is a gaseous messenger and serves as an important neuromodulator in central nervous system. In the current study, we investigated the change of H2S and cystathionine β-synthase (CBS), an H2S-synthesizing enzyme at different time points of reperfusion following global cerebral ischemia in rats, and the effect of exogenous H2S on global cerebral ischemia-reperfusion injury. First, we used global cerebral ischemia-reperfusion model by occlusion of bilateral common carotid arteries and vertebral arteries. Next, we measured H2S levels in the hippocampus, cortex and plasma, the activity of H 2S-synthesizing enzymes and expression of CBS mRNA and protein in the hippocampus and cortex at 12 h, 24 h, 48 h, 72 h and 7 days of reperfusion following 15 min cerebral ischemia. Second, we pretreated rats with different doses of sodium hydrogen sulfide (NaHS), an H2S donor and observed its effect on neuronal injury induced by 7 days of reperfusion after 15 min global cerebral ischemia. We found that when compared to sham group the amount of H2S in the hippocampus was increased significantly at 12 h of reperfusion after cerebral ischemia, markedly decreased at 24 h, restored to the same level as that in sham group at 48 h and maintained at 72 h and 7 days. The same change tendency in the levels of H2S was found in the cortex as described for the hippocampus. We found a similar change tendency in the activity of H2S-synthesizing enzymes, CBS mRNA and protein expression to that in the H2S level at different time points of reperfusion. Furthermore, while 180 μmol/kg NaHS pretreatment deteriorated the neuronal injury after global cerebral ischemia, 25 μmol/kg NaHS attenuated the neuronal injury. We suggest that a decrease of H2S level at 24 h of reperfusion after global cerebral ischemia may be involved in neuronal injury after cerebral ischemia and lower concentration rather than higher concentration of exogenous H2S may offer a protection against the neuronal injury induced by global cerebral ischemia-reperfusion.
AB - Hydrogen sulfide (H2S) is a gaseous messenger and serves as an important neuromodulator in central nervous system. In the current study, we investigated the change of H2S and cystathionine β-synthase (CBS), an H2S-synthesizing enzyme at different time points of reperfusion following global cerebral ischemia in rats, and the effect of exogenous H2S on global cerebral ischemia-reperfusion injury. First, we used global cerebral ischemia-reperfusion model by occlusion of bilateral common carotid arteries and vertebral arteries. Next, we measured H2S levels in the hippocampus, cortex and plasma, the activity of H 2S-synthesizing enzymes and expression of CBS mRNA and protein in the hippocampus and cortex at 12 h, 24 h, 48 h, 72 h and 7 days of reperfusion following 15 min cerebral ischemia. Second, we pretreated rats with different doses of sodium hydrogen sulfide (NaHS), an H2S donor and observed its effect on neuronal injury induced by 7 days of reperfusion after 15 min global cerebral ischemia. We found that when compared to sham group the amount of H2S in the hippocampus was increased significantly at 12 h of reperfusion after cerebral ischemia, markedly decreased at 24 h, restored to the same level as that in sham group at 48 h and maintained at 72 h and 7 days. The same change tendency in the levels of H2S was found in the cortex as described for the hippocampus. We found a similar change tendency in the activity of H2S-synthesizing enzymes, CBS mRNA and protein expression to that in the H2S level at different time points of reperfusion. Furthermore, while 180 μmol/kg NaHS pretreatment deteriorated the neuronal injury after global cerebral ischemia, 25 μmol/kg NaHS attenuated the neuronal injury. We suggest that a decrease of H2S level at 24 h of reperfusion after global cerebral ischemia may be involved in neuronal injury after cerebral ischemia and lower concentration rather than higher concentration of exogenous H2S may offer a protection against the neuronal injury induced by global cerebral ischemia-reperfusion.
KW - Brain
KW - Hydrogen sulfide
KW - Ischemia-reperfusion
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=77955511394&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955511394&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2010.05.017
DO - 10.1016/j.brainres.2010.05.017
M3 - Article
C2 - 20478278
AN - SCOPUS:77955511394
SN - 0006-8993
VL - 1345
SP - 197
EP - 205
JO - Brain Research
JF - Brain Research
ER -