Dysregulated expression of MIC-1/PDF in human prostate tumor cells

Dev Karan, Siu Ju Chen, Sonny L. Johansson, Ajay P. Singh, Vishwas M. Paralkar, Ming Fong Lin, Surinder K. Batra

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

As a part of the study to identify genes associated with hormone-refractory stage of human prostate cancer, we have recently identified several genetic and epigenetic changes that seem to be associated with the progression of androgen-sensitive to androgen-independent prostate tumor cells. In the present study, we report a novel gene, macrophage inhibitory cytokine-1 (MIC-1) also known as prostate derived factor (PDF), that was highly expressed in androgen-independent LNCaP-C81 cells and its metastatic variant LNCaP-Ln3 compared to androgen-sensitive LNCaP-C33 cells. The MIC-1/PDF expression was dysregulated (very low to non-detectable) in the androgen-independent PC3 and DU145 cells. Interestingly, serum factors demonstrated a differential regulation of MIC-1/PDF in the androgen-sensitive and the androgen-independent cells of LNCaP cells. Immunohistochemical analysis on 15 prostatic adenocarcinomas showed a weak staining in the benign prostatic glandular area (intensity score 2.38±0.25; n=13), while the immunoreactivity was significantly stronger (p<0.05) in areas of adenocarcinoma (score 7.33±0.88; n=15). Altogether, these data suggest that the serum factors (including androgens and cytokines) might contribute to the regulation of the MIC-1/PDF gene that seems to be associated with the progression of prostate cancer.

Original languageEnglish (US)
Pages (from-to)598-604
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume305
Issue number3
DOIs
StatePublished - Jun 6 2003

Keywords

  • Immunohistochemistry
  • LNCaP cell model
  • MIC-1/PDF
  • Prostate cancer

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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