Abstract
Purpose: To develop and evaluate diagnostic tools for early detection of wear particle-induced orthopaedic implant loosening.
Methods: N-(2-Hydroxypropyl)methacrylamide (HPMA) copolymer was tagged with a near infrared dye and used to detect the inflammation induced by polymethylmethacrylate (PMMA) particles in a murine peri-implant osteolysis model. It was established by inserting an implant into the distal femur and challenging with routine PMMA particles infusion. The osteolysis was evaluated by micro-CT and histological analysis at different time points.
Results: Significant peri-implant osteolysis was found 3-month post PMMA particle challenge by micro-CT and histological analysis. At 1-month post challenge, when there was no significant peri-implant bone loss, the HPMA copolymer-near infrared dye conjugate was found to specifically target the femur with PMMA particles deposition, but not the contralateral control femur with phosphate buffered saline (PBS) infusion.
Conclusion: The results from this study demonstrate the feasibility of utilizing the macromolecular diagnostic agent to detect particle-induced peri-implant inflammation prior to the development of detectable osteolysis. Recognition of this early pathological event would provide the window of opportunity for prevention of peri-implant osteolysis and subsequent orthopaedic implant failure.
Original language | English (US) |
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Pages (from-to) | 2086-2094 |
Number of pages | 9 |
Journal | Pharmaceutical Research |
Volume | 31 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2014 |
Keywords
- ELVIS
- HPMA copolymer
- aseptic implant loosening
- early diagnosis
- inflammation targeting
ASJC Scopus subject areas
- Biotechnology
- Molecular Medicine
- Pharmacology
- Pharmaceutical Science
- Organic Chemistry
- Pharmacology (medical)