Early growth response-1 gene: Potential radiation response gene marker in prostate cancer

Mansoor M. Ahmed, Damodaran Chendil, Subodh Lele, Kolaparthi Venkatasubbarao, Swatee Dey, Marylynn Ritter, Randall G. Rowland, Mohammed Mohiuddin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

This study was undertaken to determine whether the transcription factor EGR-1 expression: (1) in the primary tumor, correlates with radiation response in terms of complete local tumor control with no evidence of disease or recurrence and no evidence of metastasis; (2) in the postirradiated biopsies correlates with residual tumor; and (3) correlates with the expression of Egr-1 target genes such as TP53, pRB, and Bax. The authors analyzed: (1) 25 pretreated surgically resected paraffin-embedded primary adenocarcinomas of the prostate for the presence of EGR-1 expression and mutation, and correlated this with clinical endpoints such as serum prostate-specific antigen levels and current clinical status; (2) 27 postirradiated biopsies of prostate for the presence of EGR-1 expression, and correlated these findings to the residual tumor status; and (3) 12 prospective prostate tumor specimens for EGR-1 expression and its target genes. EGR-1 expression was determined by immunohistochemistry and mutations were screened in two regions of the Egr-1 gene (trinucleotide AGC repeats in transactivation domain [TD] and poly A tract in 3′UTR) by polymerase chain reaction-single strand conformational polymorphism analysis. Of 25 patients, 18 patients showed expression of EGR-1. EGR-1 overexpression correlated with treatment failure. No correlation with EGR-1 overexpression and its target genes was found, which may indirectly suggest that overexpressed EGR-1 may lack transactivation function. In summary, EGR-1 overexpression in the mutant form may provide an indication of clinical failure (local recurrence or metastasis).

Original languageEnglish (US)
Pages (from-to)500-505
Number of pages6
JournalAmerican Journal of Clinical Oncology: Cancer Clinical Trials
Volume24
Issue number5
DOIs
StatePublished - 2001

Keywords

  • Apoptosis
  • EGR-1
  • Gene overexpression
  • Prostate cancer
  • Radiation
  • Radiation resistance

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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