Early steps of V(D)J rearrangement: Insights from biochemical studies of RAG-RSS complexes

Patrick C. Swanson, Sushi Kumar, Prafulla Raval

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations

Abstract

V:(D)J recombinationis initiated by the synapsis and cleavage of a complementary(12123) pair of recombination signalsequences (RSSs) by the RAG1 and RAG2 proteins. Our understanding of these processes has been greadyaided by the development of in vitro biochemicalassays of RAG binding and cleavage activity. Accumulating evidencesuggests that synapticcomplexassembly occursin a step-wise manner and that the RAG proteins catalyze RSS cleavage by mechanisms similar to those used by bacterial transposases. In this chapter we will reviewthe molecularmechanisms of RAG synapticcomplexassembly and 12123-regulated RSS cleavage, focusingon recent advances that shed newlight on theseprocesses.

Original languageEnglish (US)
Title of host publicationV(D)J Recombination
EditorsPierre Ferrier
Pages1-15
Number of pages15
DOIs
StatePublished - 2009

Publication series

NameAdvances in Experimental Medicine and Biology
Volume650
ISSN (Print)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Swanson, P. C., Kumar, S., & Raval, P. (2009). Early steps of V(D)J rearrangement: Insights from biochemical studies of RAG-RSS complexes. In P. Ferrier (Ed.), V(D)J Recombination (pp. 1-15). (Advances in Experimental Medicine and Biology; Vol. 650). https://doi.org/10.1007/978-1-4419-0296-2_1