Ebola virus can be effectively neutralized by antibody produced in natural human infection

Toshiaki Maruyama, Luis L. Rodriguez, Peter B. Jahrling, Anthony Sanchez, Ali S. Khan, Stuart T. Nichol, C. J. Peters, Paul W.H.I. Parren, Dennis R. Burton

Research output: Contribution to journalArticle

223 Scopus citations

Abstract

The activity of antibodies against filoviruses is poorly understood but has important consequences for vaccine design and passive prophylaxis. To investigate this activity, a panel of recombinant human monoclonal antibodies to Ebola virus antigens was isolated from phage display libraries constructed from RNA from donors who recovered from infection in the 1995 Ebola virus outbreak in Kikwit, Democratic Republic of Congo. Antibodies reactive with nucleoprotein (NP), envelope glycoprotein (GP), and secreted envelope glycoprotein (sGP) were characterized by immunofluorescence and radioimmunoprecipitation assays. Four antibodies reacting strongly with sGP and weakly with GP and two antibodies reacting with NP were not neutralizing. An antibody specific for GP neutralized Ebola virus to 50% at 0.4 μg/ml as the recombinant Fab fragment and to 50% at 0.3 μg/ml (90% at 2.6 μg/ml) as the corresponding whole immunoglobulin G1 molecule. The studies indicate that neutralizing antibodies are produced in infection by Ebola virus although probably at a relatively low frequency. The neutralizing antibody may be useful in vaccine design and as a prophylactic agent against Ebola virus infection.

Original languageEnglish (US)
Pages (from-to)6024-6030
Number of pages7
JournalJournal of virology
Volume73
Issue number7
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Maruyama, T., Rodriguez, L. L., Jahrling, P. B., Sanchez, A., Khan, A. S., Nichol, S. T., Peters, C. J., Parren, P. W. H. I., & Burton, D. R. (1999). Ebola virus can be effectively neutralized by antibody produced in natural human infection. Journal of virology, 73(7), 6024-6030. https://doi.org/10.1128/jvi.73.7.6024-6030.1999