@article{33491973718644218641fd408f3264d5,
title = "Ecdysone-Induced 3D Chromatin Reorganization Involves Active Enhancers Bound by Pipsqueak and Polycomb",
abstract = "Gutierrez-Perez et al. show that BTB domain-containing isoforms of Pipsqueak associate with architectural proteins, whereas Psq lacking BTB colocalizes with Polycomb. Induction of differentiation by the hormone 20-hydroxyecdysone results in recruitment of the ecdysone receptor and Psq lacking BTB to enhancers and establishment of interactions with promoters of activated genes.",
keywords = "CTCF, GAF, HiChIP, POZ/BTB, Pipsqueak, architectural protein, chromatin, epigenetics, hormone, transcription",
author = "Irene Gutierrez-Perez and Rowley, {M. Jordan} and Xiaowen Lyu and Viviana Valadez-Graham and Vallejo, {Diana M.} and Esther Ballesta-Illan and Lopez-Atalaya, {Jose P.} and Isaac Kremsky and Esther Caparros and Corces, {Victor G.} and Maria Dominguez",
note = "Funding Information: We thank I. Oliveira, M.C. Martinez-Moratalla, and L. Mira for technical assistance; I. Gutierrez-Garcia and J. Bolivar for the UAS-Psq L transgenic line; the Bloomington Drosophila Stock Center ( NIH P40OD018537 ) for fly stocks; and the Vienna Drosophila Resource Center (VDRC, https://stockcenter.vdrc.at/control/main ) for transgenic RNAi fly stocks. We also thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and especially Drs. Angela Jones and Braden Boone, for help in performing Illumina sequencing of samples. I.G.-P. was supported by a predoctoral FPI fellowship from the Spanish Ministry of Economy and Competitiveness (MINECO) ( BES- 2010-030958 ). M.J.R. was supported by NIH Pathway to Independence Award NIGMS K99GM127671 . This work was funded by the Fundaci{\'o}n Bot{\'i}n Grant, the Generalitat Valenciana ( PROMETEO/2017/146 ), the Fundaci{\'o}n Cientifica Asociaci{\'o}n Espa{\~n}ola Contra el C{\'a}ncer (AECC) ( CICPF16001DOM{\'I} ), the Spanish Ministry of Economy and Competitiveness (MINECO) ( BFU2015-64239-R ), and cofinanced by the European Regional Development Fund (ERDF) and the Spanish State Research Agency through the “Severo Ochoa” Program for Centers of Excellence in R&D ( SEV-2013-0317 ) to M.D. This work was also supported by Pathway to Independence Award K99/R00 GM127671 (to M.J.R.) and U.S. Public Health Service Award R01 GM035463 (to V.G.C.) from the NIH . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding Information: We thank I. Oliveira, M.C. Martinez-Moratalla, and L. Mira for technical assistance; I. Gutierrez-Garcia and J. Bolivar for the UAS-PsqL transgenic line; the Bloomington Drosophila Stock Center (NIH P40OD018537) for fly stocks; and the Vienna Drosophila Resource Center (VDRC, https://stockcenter.vdrc.at/control/main) for transgenic RNAi fly stocks. We also thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and especially Drs. Angela Jones and Braden Boone, for help in performing Illumina sequencing of samples. I.G.-P. was supported by a predoctoral FPI fellowship from the Spanish Ministry of Economy and Competitiveness (MINECO) (BES-2010-030958). M.J.R. was supported by NIH Pathway to Independence Award NIGMS K99GM127671. This work was funded by the Fundaci?n Bot?n Grant, the Generalitat Valenciana (PROMETEO/2017/146), the Fundaci?n Cientifica Asociaci?n Espa?ola Contra el C?ncer (AECC) (CICPF16001DOM?), the Spanish Ministry of Economy and Competitiveness (MINECO) (BFU2015-64239-R), and cofinanced by the European Regional Development Fund (ERDF) and the Spanish State Research Agency through the ?Severo Ochoa? Program for Centers of Excellence in R&D (SEV-2013-0317) to M.D. This work was also supported by Pathway to Independence Award K99/R00 GM127671 (to M.J.R.) and U.S. Public Health Service Award R01 GM035463 (to V.G.C.) from the NIH. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. I.G.-P. M.J.R. V.G.C. and M.D. designed the project and wrote the manuscript. I.G.-P. performed Drosophila work, ChIP-seq and bioinformatic analysis, and coIPs. X.L. and V.V.-G. performed HiChIP experiments. E.C. E.B.-I. and D.M.V. helped perform experiments. M.J.R. performed major bioinformatics analysis, with contributions from J.L.-A. and I.K. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = sep,
day = "3",
doi = "10.1016/j.celrep.2019.07.096",
language = "English (US)",
volume = "28",
pages = "2715--2727.e5",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "10",
}