Effect of a collagen substrate on the growth and development of normal and tumorigenic rat urothelial cells

F. J. Chlapowski, B. D. Minsky, J. B. Jacobs, S. M. Cohen

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The growth and morphology of 4 tumorigenic rat urothelial cell lines grown on collagen-coated nylon discs was characterized and compared to normal cells. In contrast to cells cultured on a plastic substrate with or without a thin 'nonporous' collagen coating, tumor cells grown on porous collagen-coated nylon discs: 1) grew to greater protein densities; 2) formed tissue structures characteristic for the type of tumor they developed upon back-transplantation; and 3) could be grown and cultured indefinitely without subculturing. Thus, similarly to normal urothelial stratification and differentiation in vitro, tumorigenic cells apparently require a 'porous' collagen substrate to allow differentiation analogous to that observed in vivo.

Original languageEnglish (US)
Pages (from-to)1211-1216
Number of pages6
JournalJournal of Urology
Volume130
Issue number6
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Urology

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