Effect of base-pair stability on the melting of superhelical DNA

A. V. Gagua; B. N. Belintsev; Yu L. Lyubchenko

doi:10.1038/294662a0

Effect of base-pair stability on the melting of superhelical DNA

A. V. Gagua, B. N. Belintsev, Yu L. Lyubchenko

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

It is well established that the structure^1,2 as well as the stability of the DNA duplex is affected by supercoiling. Supercoiled DNA has been shown to possess increased stability and is thus characterized by an elevated melting temperature as well as a broader melting transition. Thermal melting analysis has been the most widely used method for studying the structural and dynamic properties of complex DNA species. Here we report a remarkable attribute of supercoiled DNA, that the width of the melting transition of superhelical DNA does not depend appreciably on the relative stabilities of its A·T and G·C base pairs.

Original language	English (US)
Pages (from-to)	662-663
Number of pages	2
Journal	Nature
Volume	294
Issue number	5842
DOIs	https://doi.org/10.1038/294662a0
State	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1038/294662a0

Cite this

@article{c14568795f3b4f8596a9d4e752c0dc4f,

title = "Effect of base-pair stability on the melting of superhelical DNA",

abstract = "It is well established that the structure1,2 as well as the stability of the DNA duplex is affected by supercoiling. Supercoiled DNA has been shown to possess increased stability and is thus characterized by an elevated melting temperature as well as a broader melting transition. Thermal melting analysis has been the most widely used method for studying the structural and dynamic properties of complex DNA species. Here we report a remarkable attribute of supercoiled DNA, that the width of the melting transition of superhelical DNA does not depend appreciably on the relative stabilities of its A·T and G·C base pairs.",

author = "Gagua, {A. V.} and Belintsev, {B. N.} and Lyubchenko, {Yu L.}",

year = "1981",

doi = "10.1038/294662a0",

language = "English (US)",

volume = "294",

pages = "662--663",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Research",

number = "5842",

}

TY - JOUR

T1 - Effect of base-pair stability on the melting of superhelical DNA

AU - Gagua, A. V.

AU - Belintsev, B. N.

AU - Lyubchenko, Yu L.

PY - 1981

Y1 - 1981

N2 - It is well established that the structure1,2 as well as the stability of the DNA duplex is affected by supercoiling. Supercoiled DNA has been shown to possess increased stability and is thus characterized by an elevated melting temperature as well as a broader melting transition. Thermal melting analysis has been the most widely used method for studying the structural and dynamic properties of complex DNA species. Here we report a remarkable attribute of supercoiled DNA, that the width of the melting transition of superhelical DNA does not depend appreciably on the relative stabilities of its A·T and G·C base pairs.

AB - It is well established that the structure1,2 as well as the stability of the DNA duplex is affected by supercoiling. Supercoiled DNA has been shown to possess increased stability and is thus characterized by an elevated melting temperature as well as a broader melting transition. Thermal melting analysis has been the most widely used method for studying the structural and dynamic properties of complex DNA species. Here we report a remarkable attribute of supercoiled DNA, that the width of the melting transition of superhelical DNA does not depend appreciably on the relative stabilities of its A·T and G·C base pairs.

UR - http://www.scopus.com/inward/record.url?scp=0019825462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019825462&partnerID=8YFLogxK

U2 - 10.1038/294662a0

DO - 10.1038/294662a0

M3 - Article

C2 - 6458769

AN - SCOPUS:0019825462

SN - 0028-0836

VL - 294

SP - 662

EP - 663

JO - Nature

JF - Nature

IS - 5842

ER -

Effect of base-pair stability on the melting of superhelical DNA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this