TY - JOUR
T1 - Effect of blood group determinants on binding of human salivary mucous glycoproteins to influenza virus
AU - Boat, Thomas F.
AU - Davis, James
AU - Stern, Robert C.
AU - Cheng, Pi Wan
N1 - Funding Information:
T:he authors wish to thank Dr. Lansing Hoskins for the a-galactosidase used in this study. This work was supported in part by grants HL14844 and HR52957 from the United States Public Health Service and a grant from the Health Fund of Cleveland.
PY - 1978/4/19
Y1 - 1978/4/19
N2 - We have demonstrated that the inhibitor of influenza B virus hemagglutination in human saliva is inactivated by neuraminidase and is associated with the mucous glycoprotein fraction (blood group substance) of this secretion. Inhibitory activity of saliva was found to be roughly proportional to its sialic acid content (r = 0.456). However, the minimal quantity of salivary sialic acid, neutral sugar, or blood group antigen required to inhibit virus hemagglutinatino was greater of secretors of A and B than for secretors of H and Lea blood group substances. Removal of terminal galactose from blood group B substance with α-galactosidase markedly decreased blod group B activity but increased blood group H and virus hemagglutination inhibitory activities of this glycoprotein. These data suggest that terminal α-linked galactose and, probably, N-acetyl-galactosamine interfere with access of influenza virus to binding sites onn oligosaccharide chains of the mucous glycoprotein.
AB - We have demonstrated that the inhibitor of influenza B virus hemagglutination in human saliva is inactivated by neuraminidase and is associated with the mucous glycoprotein fraction (blood group substance) of this secretion. Inhibitory activity of saliva was found to be roughly proportional to its sialic acid content (r = 0.456). However, the minimal quantity of salivary sialic acid, neutral sugar, or blood group antigen required to inhibit virus hemagglutinatino was greater of secretors of A and B than for secretors of H and Lea blood group substances. Removal of terminal galactose from blood group B substance with α-galactosidase markedly decreased blod group B activity but increased blood group H and virus hemagglutination inhibitory activities of this glycoprotein. These data suggest that terminal α-linked galactose and, probably, N-acetyl-galactosamine interfere with access of influenza virus to binding sites onn oligosaccharide chains of the mucous glycoprotein.
UR - http://www.scopus.com/inward/record.url?scp=0017807144&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017807144&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(78)90441-5
DO - 10.1016/0304-4165(78)90441-5
M3 - Article
C2 - 638205
AN - SCOPUS:0017807144
SN - 0304-4165
VL - 540
SP - 127
EP - 133
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 1
ER -