TY - JOUR
T1 - Effect of cell density on androgen regulation of the mRNA level of human prostatic acid phosphatase
AU - Lin, Ming Fong
AU - Garcia-Arenas, Renee
N1 - Funding Information:
The author thankst he Word ProcessingP ool of the Norris ComprehensiveC ancer at the USC School of Medicine for secretarial assistance in manuscript preparation,a nd Fen-Fen Lin for excellentt echnical supports.T his investigationw as supportedi n part by a grant from the National Cancer Institute CA52112.
PY - 1994/3
Y1 - 1994/3
N2 - The expression of human prostatic acid phosphatase (PAcP), a differentiation antigen of prostate epithelial cells, has been proposed to be regulated by androgen. We investigated this regulatory mechanism at the post-transcriptional level in LNCaP human prostate carcinoma cells, the only cultured cells that express PAcP. Our results demonstrated that the level of PAcP mRNA decreased when the cell density increased. Further, 5α-dihydrotestosterone, an active form of the endogenous androgen, stimulated the PAcP mRNA level in low-density cells; while, caused a decrease in high-density cells. Thus, in LNCaP human prostate carcinoma cells, cell density could modulate PAcP expression at the mRNA level including androgen regulation.
AB - The expression of human prostatic acid phosphatase (PAcP), a differentiation antigen of prostate epithelial cells, has been proposed to be regulated by androgen. We investigated this regulatory mechanism at the post-transcriptional level in LNCaP human prostate carcinoma cells, the only cultured cells that express PAcP. Our results demonstrated that the level of PAcP mRNA decreased when the cell density increased. Further, 5α-dihydrotestosterone, an active form of the endogenous androgen, stimulated the PAcP mRNA level in low-density cells; while, caused a decrease in high-density cells. Thus, in LNCaP human prostate carcinoma cells, cell density could modulate PAcP expression at the mRNA level including androgen regulation.
KW - Androgen regulation
KW - Cell density
KW - Prostatic acid phosphatase (Human)
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U2 - 10.1016/0303-7207(94)90025-6
DO - 10.1016/0303-7207(94)90025-6
M3 - Article
C2 - 8206318
AN - SCOPUS:0028213320
SN - 0303-7207
VL - 99
SP - R21-R24
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 2
ER -