Abstract
Effects of different C/N (NO 2 - N) ratios on nitrogen removal and microbial community structure were investigated using an anaerobic baffled reactor (ABR). Results indicated that the C/N ratio exerted an important effect on nitrogen removal in the anammox-coupling-denitrification process associated with the ABR. When the C/N ratio was 1.29, the ABR could achieve the highest total nitrogen (TN) removal efficiency of 99.9%. Most of TN was removed in the 1st and 2nd compartment, accounting for about 81.0-97.6% of total TN removal. The nitrogen removal resulted from the interaction among anammox, heterotrophic denitrificans, and other microbes within the ABR. The contribution of anammox to nitrogen removal varied from 6.8% to 32.4%. High-throughput MiSeq sequencing analyses revealed that the C/N ratio was one of the most important factors regulating the microbial community structure, and the predominant phylum changed from Proteobacteria to Chloroflexi with the elevated C/N ratio. In addition, the Candidatus Brocadia was the major anammox bacterium, and its percentage varied from 1.0-2.9% at day 9 to 2.8-9.1% at day 46.
Original language | English (US) |
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Pages (from-to) | 2338-2348 |
Number of pages | 11 |
Journal | Water Science and Technology |
Volume | 78 |
Issue number | 11 |
DOIs | |
State | Published - Dec 28 2018 |
Keywords
- Anaerobic baffled reactor (ABR)
- Anammox
- C/N ratio
- Denitrification
- MiSeq sequencing analysis
- Microbial community structure
ASJC Scopus subject areas
- Environmental Engineering
- Water Science and Technology